Rabbit Anti-Human ANXA3 (a.a. 51-150) Polyclonal antibody

The aim of the study was to investigate the effect of 3, 10, and 20% of platelet-rich rat plasma (PRP) on the morphological characteristics of chondrocyte-like cells of the rat nucleus pulposus (NP) and the expression of chondrogenic genes ACAN, COLII, GPC3, MGP, PTN, ANXA3, and VIM in vitro. A dose-dependent increase of NP cell viability was observed in cultures supplemented with 3, 10, and 20% PRP. Platelets showed tropicity to blast-like cells in culture and formed adhesion zones. Differentiation of blast-like cells was observed and dividing cells were identified on day 14 of cultivation with 20% PRP. PRP prevented the decrease of chondrogenic gene expression in a dose-dependent manner, with 20% PRP being the most efficient. Expression of the majority of chondrogenic genes studied was observed in these cultures: the expression of COLII increased 2.9 times (P = 0.002); that of VIM, 2.1 times (P = 0.0003); that of ANXA3, 2.3times (P = 0.0003); that of GPC3, 1.4 times (P = 0.0011); that of PTN, 1.6 times (P = 0.0017); and that of ACAN, 1.2 times (P = 0.0606).

Annexin A3 (ANXA3) has been found to play important roles in cancer progression, metastasis, and drug resistance; however, its role in hepatocellular carcinoma (HCC) remains unknown. In this study, we investigated the expression level, clinical significance and biologic function of ANXA3 in HCC. Real-time quantitative reverse transcriptase-polymerase chain reaction, western blotting and immunohistochemical staining were used to examine ANXA3 expression levels in HCC tumor tissue, and its correlation with the clinicopathological features and prognosis of HCC patients was analyzed. The biological functions of ANXA3 in cell proliferation, migration, invasion, and resistance to chemotherapy were also investigated. ANXA3 expression was significantly increased in HCC tissues as compared with adjacent non-tumorous tissues. Elevated ANXA3 expression was associated with tumor size, number of lesions, tumor stage, and poor prognosis. In hepatoma cell lines, exogenous ANXA3 transduction promoted the tumorigenic activity and metastatic potential of tumor cells. Small interfering RNA silencing of ANXA3 inhibited these processes. In addition, in vitro and in vivo experiments revealed that ANXA3 overexpression enhanced resistance to chemotherapy. Taken together, our findings reveal that ANXA3 might play an important role in HCC progression and chemoresistance, and could serve as a novel prognostic marker and therapeutic target for HCC. (c) 2013 Wiley Periodicals, Inc.