Rabbit Anti-Human AAK1 (internal) Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human; Mouse; Rat

Immunogen

A synthesized peptide derived from human AAK1, corresponding to a region within the internal amino acids.

Conjugate

Application Notes

WB 1:500-1:1000
IF/ICC 1:100-1:500

General Notes

This product (Catalog No. DPABH-27581) is a rabbit-derived polyclonal antibody raised against human AAK1. This antibody exhibits cross-reactivity with AAK1 from mouse and rat. It has been affinity purified by Protein A. Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.
DPABH-27581 is suitable for use in WB 1 (500-1:1000), IF/ICC 1 (100-1:500) applications.
DPABH-27581 is supplied as a liquid formulation in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Entrez Gene ID

UniProt ID