Anti-2,2,7-Trimethylguanosine monoclonal antibody [Agarose] (DMABT-H21925)

Mouse anti-2,2,7-Trimethylguanosine monoclonal antibody for IP, WB Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
L232
Species Reactivity
N/A
Immunogen
2,2,7-trimethylguanosine
Conjugate
Agarose

Applications


Application Notes
Affinity Purification Immunoprecipitation (15 μl beads/sample)
Immunoprecipitation (1-2 μg/ml)
Immunocytochemistry Paraffin Sections Note: Also recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
2,2,7-trimethylguanosine antibody; Mouse Anti-2,2,7-Trimethylguanosine Monoclonal Antibody; Anti-2,2,7-Trimethylguanosine Monoclonal Antibody; 2,2,7-Trimethylguanosine Monoclonal Antibody Mouse Anti-2,2,7-Trimethylguanosine MAb; Anti-2,2,7-Trimethylguanos

Product Background


Antigen Description
The U small nuclear ribonucleoprotein particles (snRNPs) are present in all eukaryotes studied thus far and are involved in the splicing of RNA molecules. snRNPs are composed of several proteins and one or two RNA molecules. The RNA molecules (snRNAs) contain a unique 5" terminal cap structure containing the nucleoside 2,2,7-trimethylguanosine. The 70 kDa protein component of U1 snRNP is cleaved during apoptosis, perhaps by a caspase. Monoclonal antibodies to the 5" cap structure react only to snRNAs.

Citations


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References


Rasmussen, TP; Culbertson, MR; et al. Analysis of yeast trimethylguanosine-capped RNAs by midwestern blotting. GENE 182:89-96(1996).
BACH, M; BRINGMANN, P; et al. PURIFICATION OF SMALL NUCLEAR RIBONUCLEOPROTEIN-PARTICLES WITH ANTIBODIES AGAINST MODIFIED NUCLEOSIDES OF SMALL NUCLEAR RNAS. METHODS IN ENZYMOLOGY 181:232-257(1990).

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