Human Angiotensin II Receptor 2 ELISA kit

Name: Human Angiotensin II Receptor 2 ELISA kit
SKU: DEIA-BJ982
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Species Reactivity

Human

Detection Method

Competitive ELISA

Intended Use

Human Angiotensin II Receptor 2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the Angiotensin II Receptor 2. This ELISA kit is for research use only, not for therapeutic or diagnostic applications.

Contents of Kit

1. MICROTITER PLATE: 96 wells
2. ENZYME CONJUGATE: 6.0 mL or 10 ml
3. STANDARD A-F: 1 vial each
4. SUBSTRATE A: 6 mL
5. SUBSTRATE B: 6 mL
6. STOP SOLUTION: 6 mL
7. WASH SOLUTION (100 x): 10 mL
8. BALANCE SOLUTION: 3 mL

Storage

All components of this kit are stable at 2-8°C until the kit's expiration date.

Detection Range

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

SINGLE NUCLEOTIDE POLYMORPHISMS OF ADD1 alpha, AGT, AGTR1 AND AGTR2 GENES IN DIFFERENT ETHNIC GROUPS OF YAKUTIA ARCTIC ZONE RESIDENTS, SUFFERING FROM ARTERIAL HYPERTENSION

YAKUT MEDICAL JOURNAL

Authors: Komzin, K. V.; Petrova, P. G.; Strekalovskaya, A. A.; Samsonov, S. N.; Parshina, S. S.; Andreeva, A. A.

Abstract

The article presents the results of the study of the frequencies of occurrence of single nucleotide polymorphisms of the genes ADD1 alpha (1378 G> T), AGT (704 T> C and 521 C> T), AGTR1 (1166 A> C) and AGTR2 (1675 G> A) groups of residents of the Arctic zone of the RS (Ya), suffering from essential arterial hypertension. Subjects of the research were represented by the most widespread ethnic groups in the territory, including the Slavs, Yakuts, Evens and Evenks. To reveal the above mentioned polymorphisms, a real-time PCR method was used with detection of the melting temperature of duplexes. In the course of the study statistically significant differences between the study groups were identified by the points ADD1 alpha 1378 G> T; AGT 521 C> T and AGTR1 1166 A> C.

Association of Polymorphisms in the Genes of Angiotensinogen and Angiotensin Receptors With Risk for Basal Cell Carcinoma

ANTICANCER RESEARCH

Authors: Papaggelopoulos, John; Angelopoulou, Antonia; Avgoustidis, Dimitris; Koronellos, Nikolas; Derka, Spyridoula; Vassiliou, Stavros; Yapijakis, Christos

Abstract

Background/Aim: Basal cell carcinoma (BCC) has been genetically associated with an increased expression of angiotensin-converting enzyme (ACE), an important factor of the renin-angiotensin system which produces vasoconstrictor angiotensin II. Other factors of this system include angiotensinogen (AGT) and angiotensin receptors AGTR1, AGTR2. We investigated the possible association of BCC with genetic variability in the AGT, AGTR1 and AGTR2 genes. Materials and Methods: DNA samples of 190 Greeks were studied, including 91 patients with BCC and 99 matched healthy controls. Molecular genotyping of patients and controls was performed for the polymorphisms AGT M235T, AGTR1 A1166C and AGTR2 G1675A. Results: The mutant T allele that increases AGT gene expression was detected in two-fold increased frequency in BCC patients in comparison to healthy controls (p < 0.001). On the contrary, no significant difference was observed in AGTR1 and AGTR2 variants between patients and controls. Conclusion: Increased expression of AGT may be associated with BCC.