Adrenaline - Noradrenaline - Dopamine ELISA Kit

Sample

Plasma, urine

Species Reactivity

Human

Detection Method

cELISA

Intended Use

For the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine in plasma and urine. For Research Use Only. Not For Use In Diagnostic Procedures.

Contents of Kit

1. Adhesive Foil, Ready to use: Adhesive foils in a resealable pouch. 3 × 4 foils
2. Wash Buffer Concentrate 50×: Buffer with a non-ionic detergent at physiological pH. 3 × 20 mL/vial, light purple cap
3. Enzyme Conjugate, Ready to use: Goat anti-rabbit immunoglobulin, conjugated with peroxidase. 3 × 12 mL/vial, red cap
4. Substrate, Ready to use: Chromogenic substrate containing tetramethylbenzidine, substrate buffer and hydrogen peroxide. 3 × 12 mL/vial, black cap
5. Stop Solution, Ready to use: 0.25 M sulfuric acid. 3 × 12 mL/vial, light grey cap.
6. A drenaline Microtiter Strips, Ready to use: 1 × 96 well (12×8) antigen precoated microwell plate in a resealable blue pouch with desiccant
7. Noradrenaline Microtiter Strips, Ready to use: 1 × 96 well (12×8) antigen precoated microwell plate in a resealable yellow pouch with desiccant
8. Dopamine Microtiter Strips, Ready to use: 1 × 96 well (12×8) antigen precoated microwell plate in a resealable green pouch with desiccant
9. Adrenaline Antiserum, Ready to use: Rabbit anti-adrenaline antibody, blue colored. 1 × 6 mL/vial, blue cap
10. Noradrenaline Antiserum, Ready to use: Rabbit anti-noradrenaline antibody, yellow colored. 1 × 6 mL/vial, yellow cap
11. Dopamine Antiserum, Ready to use: Rabbit anti-dopamine antibody, green colored. 1 × 6 mL/vial, dark green cap
12. Adjustment Buffer, Ready to use: TRIS buffer. 2 × 4 mL/vial, green cap
13. Acylation Buffer, Ready to use: Buffer with light alkaline pH for the acylation. 1 × 20 mL/vial, white cap
14. Acylation Reagent, Ready to use: Acylation reagent in DMSO. 1 × 3 mL/vial, white cap
15. Assay Buffer, Ready to use: 1M hydrochloric acid and a non-mercury preservative. 1 × 6 mL/vial, light grey cap
16. Coenzyme, Ready to use: S-adenosyl-L-methionine. 1 × 4 mL/vial, purple cap
17. Enzyme, Lyophilized: Catechol-O-methyltransferase. 6 vials, pink cap
18. Extraction Buffer, Ready to use: Buffer containing carbonate. 1 × 6 mL/vial, brown cap
19. Extraction Plate, Ready to use: 2 × 48 well plates coated with boronate affinity gel in a resealable pouch
20. Hydrochloric Acid, Ready to use: 0.025 M Hydrochloric Acid, yellow colored. 1 × 20 mL/vial, dark green cap
21. STANDARD A: 4mL
ADR 0ng/mL (0nmol/L), NAD 0ng/mL (0nmol/L), DOP 0ng/mL (0nmol/L).
22. STANDARD B: 4mL
ADR 1ng/mL (5.5nmol/L), NAD 5ng/mL (30nmol/L), DOP 10ng/mL (65nmol/L).
23. STANDARD C: 4mL
ADR 4ng/mL (22nmol/L), NAD 20ng/mL (118nmol/L), DOP 40ng/mL (261nmol/L).
24. STANDARD D: 4mL
ADR 15ng/mL (82nmol/L), NAD 75ng/mL (443nmol/L), DOP 150ng/mL (980nmol/L).
25. STANDARD E: 4mL
ADR 50ng/mL (273nmol/L), NAD 250ng/mL (1478nmol/L), DOP 500ng/mL (3265nmol/L).
26. STANDARD F: 4mL
ADR 200ng/mL (1092nmol/L), NAD 1000ng/mL (5910nmol/L), DOP 2000ng/mL (13060nmol/L).
27. STANDARD A/B: 4mL
ADR -ng/mL (-nmol/L), NAD -ng/mL (-nmol/L), DOP 4.5ng/mL (29nmol/L).
28. CONTROL 1: 4mL. Refer to QC-Report for expected value and acceptable range!
29. CONTROL 2: 4mL. Refer to QC-Report for expected value and acceptable range!
Conversion: Adrenaline (ng/mL) × 5.46 = Adrenaline (nmol/L)
Noradrenaline (ng/mL) × 5.91 = Noradrenaline (nmol/L)
Dopamine (ng/mL) × 6.53 = Dopamine (nmol/L)
Content: Acidic buffer with non-mercury stabilizer, spiked with defined quantity of adrenaline, noradrenaline, and dopamine
*for the determination of dopamine in plasma the additional Standard A/B is mandatory!

Storage

Store the unopened reagents at 2 - 8 °C until the expiration date. Do not use components beyond the expiry date indicated on the kit labels. Once opened the reagents are stable for 2 months when stored at 2 – 8 °C. Once the resealable pouch has been opened, care should be taken to close it tightly with desiccant again.

Precision

Detection Limit

General Description

In humans, the catecholamines adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are neurotransmitters of the sympathetic nervous system and are involved in many physiological processes. The sympathetic nervous system sets the body to a heightened state of alert, also called the body' s fight-or-flight response. The catecholamines and their metabolites indicate the adaptation of the body to acute and chronic stress.

Standard Curve

Examples, do not use for calculation!