Human Acetaminophen ELISA Kit

Name: Human Acetaminophen ELISA Kit
SKU: DEIA-XYZ191
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Urine, whole blood, oral fluid and other forensic samples

Species Reactivity

Human

Detection Method

cELISA

Intended Use

This kit is a qualitative one-step kit designed for use as a screening device for the detection of Acetaminophen and/or their metabolites in human urine, blood or oral fluid.

Contents of Kit

1. EIA Buffer, 30 mL (ready-to-use). Phosphate buffered saline solution with bovine serum and a preservative. Provided for dilution of samples.
2. Wash Buffer Concentrate (10X), 20 mL. Phosphate buffered saline solution with a surfactant. Dilute 10 fold with deionized or ultrapure water before use. Diluted wash buffer is used to wash all unbound conjugate and samples from the plate after the conjugate incubation.
3. Substrate, 20 mL (ready-to-use). Stabilized 3, 3', 5, 5' Tetramethylbenzidine (TMB) plus Hydrogen Peroxide (H₂O₂) in a single bottle. It is used to develop the color in the wells after washing. Light Sensitive.
4. Drug-Enzyme Conjugate, 14 mL (ready-to-use). Drug-horseradish peroxidase conjugate. Do not dilute.
5. Antibody Coated Plate, A 96-well Costar plate, in strips of 8 break-away wells, coated with anti-drug antiserum. The plate is ready for use as is. Do not wash.
6. Acid Stop Solution, 14 mL (ready-to-use). 1N H₂SO₄ used to stop the enzyme reaction.
7. Qualitative QC Positive Control, 750 μL provided (synthetic human urine). Do not dilute.
8. Qualitative QC Negative Control, 750 μL provided (synthetic human urine). Do not dilute.

Storage

This kit can be used until the expiration date on the label when stored refrigerated at 2-8°C. Store controls frozen if not used within 10 days. Note: Some kits require controls to be stored frozen immediately upon receipt. Reference kit label for details.

Sensitivity

The I-50 in EIA Buffer is 402 ng/mL. In Whole Blood with a 1:5 dilution (1 part sample to 4 parts EIA buffer), the I-50 is 1,687 ng/mL. In Whole Blood with a 1:10 dilution (1 part sample to 9 parts EIA buffer), the I-50 is 2,817 ng/mL. In Whole Blood with a 1:50 dilution (1 part sample to 49 parts EIA buffer), the I-50 is 13,629 ng/mL.

The I-50 in Urine with a 1:10 dilution (1 part sample to 9 parts EIA buffer) is 8,410 ng/mL. The I-50 in Urine plus Sodium Azide with a 1:50 dilution (1 part sample to 49 parts EIA buffer) is 24,469 ng/mL.

The term I-50 is used to define the sensitivity of the test. This number is derived from a standard curve
generated with the drug in EIA Buffer. The drug concentration that shows 50% less color activity than the zerostandard is considered to be the I-50.

General Description

The Acetaminophen ELISA (Enzyme-Linked ImmunoSorbent Assay) test kit is a qualitative one-step kit designed for use as a screening device for the detection of drugs and/or their metabolites. The kit was designed for screening purposes and is intended for forensic use only. It is recommended that all suspect samples be confirmed by a quantitative method such as gas chromatography/mass spectrometry (GC/MS).

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Q & A

Ask a Question

Q: Hi, I am after ELISA kits to measure acetaminophen (or its metabolites) in mouse serum.

A: Yes, the Acetaminophen RTU kit is non-species specific so mice would be fine, and serum would work fine as well. Our technical service supervisor suggested you may need to do a similar dilution as in whole blood to negative background, so he would typically suggest starting out with a 1:5 dilution and see what that does for your test.

Q: The kit says it works with blood but does not mention serum, could you please confirm that we can use if on serum?

A: Yes, the Acetaminophen kit is non-species specific, and serum would work fine as well. Our technical service supervisor suggested you may need to do a similar dilution as in whole blood to negative background, so he would typically suggest starting out with a 1:5 dilution and see what that does for your test.

Customer Reviews

References

Use of acetaminophen for patent ductus arteriosus treatment: a single center experience

JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE

Authors: Vaidya, Ruben; Wilson, Donna; Paris, Yvonne; Madore, Laura; Singh, Rachana

Abstract

Purpose:Patent ductus arteriosus (PDA) continues to be one of the most common complications associated with preterm birth. Up to 70% of infants born before 28 weeks gestational age may require some form of medical or surgical treatment for PDA closure. Recent studies have suggested acetaminophen to be a promising new alternative to indomethacin and ibuprofen for closure of PDA with potentially fewer adverse effects. Our aim for the study was to report our experience regarding the efficacy of acetaminophen compared to indomethacin for treatment of hemodynamically significant PDA (hs-PDA) in infants born in our institution. Material and methods:Retrospective cohort study of all preterm infants born <34-week gestation with hs-PDA, treated with acetaminophen or indomethacin as the first line medication for hs-PDA. Primary outcome of successful PDA closure rate (small or no PDA) and secondary outcomes of short-term morbidities and immediate adverse events were compared between the two cohorts. Results:Of the 43 infants, 25 were treated with acetaminophen and 18 with indomethacin, as first line for hs-PDA. Successful PDA closure rate was slightly lower for acetaminophen compared to indomethacin, although statistically not significant (acetaminophen: 40% versus indomethacin: 55.5%,p = .31). No significant differences in short-term morbidities including necrotizing enterocolitis (NEC), bronchopulmonary dysplasia (BPD), late onset sepsis (LOS), retinopathy of prematurity (ROP) and intraventricular hemorrhage (IVH), or immediate side effects including oliguria, hyponatremia, elevated BUN/creatinine, thrombocytopenia were found between the two cohorts. Conclusions:Acetaminophen treatment of hs-PDA resulted in similar successful PDA closure rate compared to indomethacin in our small cohort of patients.

Ag nanoparticles anchored onto porous CuO nanobelts for the ultrasensitive electrochemical detection of dopamine in human serum

SENSORS AND ACTUATORS B-CHEMICAL

Authors: Li, Yong-Yu; Kang, Ping; Wang, Shi-Qi; Liu, Zhang-Gang; Li, Yi-Xiang; Guo, Zheng

Abstract

The potential capability to rapid, highly sensitive, and selective diagnose neurotransmitters in human serum environment is extremely crucial for clinical biology. Herein an electrochemical sensor based on Ag nano -particles anchored onto CuO porous nanobelts (Ag/CuO PNBs) was constructed for the ultrasensitive determination of dopamine (DA), which was prepared via a facile two-step cation-exchange reaction followed by in-situ thermal conversion. The Ag/CuO PNBs exhibited an excellent electrochemical sensing performance to DA. The linear range for the detection of DA was ranged from 0.04 to 10 mu M with an outstanding detection limit of 7.0 nM. Cyclic voltammograms (CVs) were employed to investigate kinetic information of multiphase electron transfer reactions, revealing that Ag nanoparticles significantly enhanced electrocatalytic activity toward DA owing to the participation of Ag(0)/Ag(I) cycle. Meanwhile, the Ag/CuO PNBs showed the significant selectivity, remarkable stability and repeatability, as well as attractive reproducibility (RSD <= 3.7 %). Furthermore, the practicality of the developed sensor has been evaluated by analyzing DA in serum-containing electrolyte, and the recovery in the range of 95.7-111.1 % was obtained. The results confirmed that the as-prepared sensor presented a significant promise for the detection of DA in real sample analysis.