ATP5E Knockout Cell Lysate (DAG-KO024)
ATP5E Knockout Cell Lysate from HeLa cell line for anti-ATP5E antibody specificity validation.
Datasheet
Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The ATP5E knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Nature
Cell Lysate
Alternative Names
ATP5E; ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit; ATP synthase subunit epsilon, mitochondrial; F(0)F(1)-ATPase; mitochondrial ATPase; H(+)-transporting two-sector ATPase
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Format
Lyophilized
Concentration
The protein concentration was determined with BCA assay.
Size
100 ug
Buffer
RIPA buffer
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
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