ASCA IgG/IgA ELISA Kit

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

Enzyme immunoassay for the quantitative measurement of IgG and IgA class anti-Saccharomyces cerevisiae antibodies (ASCA) in human serum or plasma.

Contents of Kit

Sufficient for 96 determinations
1. SORB MT 1× divisible microplate consisting of 12 modules of 8 wells each. Ready to use.
2. CAL A-F 6× 1.5 ml Calibrator A-F (0, 6.3, 12.5, 25, 50, 100 U/ml), containing serum/buffer matrix (PBS, BSA, detergent, NaN3 0.09%), yellow. Ready to use. This assay system is calibrated in relative arbitrary units, since no international reference preparation is available for this assay.
3. CONTROL 1 & 2 2× 1.5 ml Control positive (1) and negative (2), containing ASCA antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN3 0.09%), yellow. Ready to use. The concentration is specified on the certificate of analysis.
4. SAM DIL 5× 20 ml Sample Buffer, containing PBS, BSA, detergent, preservative NaN3 0.09%, yellow, 5× conc.
5. ENZ CONJ IgG 15 ml Enzyme Conjugate, containing anti-human IgG antibodies, HRP labelled, PBS, BSA, detergent, preservative ProClin 300 0.05%, light red. Ready to use.
6. ENZ CONJ IgA 15 ml Enzyme Conjugate, containing anti-human IgA antibodies, HRP labelled, PBS, BSA, detergent, preservative ProClin 300 0.05%, light red. Ready to use.
7. SUB TMB 15 ml TMB Substrate, containing 3,3', 5,5'- Tetramethylbenzidin, colorless. Ready to use.
8. STOP SOLN 15 ml Stop solution, contains acid. Ready to use.
9. WASH SOLN 50× 20 ml Wash Buffer, containing Tris, detergent, preservative NaN3 0.09%; 50× conc.
10. 1 Instruction for Use
11. 1 Certificate of Analysis

Storage

1. Store test kit at 2-8°C in the dark.
2. Do not expose reagents to heat, sun, or strong light during storage and usage.
3. Store microplate sealed and desiccated in the clip bag provided.
4. Unopened reagents are stable until expiration of the kit. See labels for individual batch.
5. Diluted Wash Solution and Sample Buffer are stable for at least 30 days when stored at 2-8°C. We recommend consumption on the same day.

Detection Range

The calculation range of this ELISA assay is
IgG: 0 - 100 U/ml
IgA: 0 - 100 U/ml

Detection Limit

Functional sensitivity was determined to be:
IgG: 0.5 U/ml
IgA: 0.5 U/ml

General Description

ASCA IgG/IgA ELISA is a test system for the quantitative measurement of IgG and IgA class antiSaccharomyces cerevisiae antibodies (ASCA) in human serum or plasma. This product is intended for professional in vitro diagnostic use only. Accurate diagnosis of inflammatory bowel disease (IBD), in particular the differentiation between the ulcerative colitis and Crohn's disease, is important for treatment and prognosis. ASCA are strongly associated to Crohn's disease. IgG as well as IgA ASCA show a high specificity for Crohn's disease, and support the differential dignosis of IBD, when combined with other serological markers and clinical tests.