Potato Andean Mottle Virus (APMoV) ELISA Kit

Name: Potato Andean Mottle Virus (APMoV) ELISA Kit
SKU: DEIAPV227
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

host plants

Intended Use

The test can be used to detect APMoV in host plants.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is very high. The virus can be consistently detected in infected plant tissues diluted at 1:2430.

General Description

Andean potato mottle virus (APMoV) is a plant pathogenic virus of the family Comoviridae. A virus with isometric, RNA-containing particles about 26 nm in diameter. It has a narrow host range, is readily transmissible by manual inoculation and is widespread in potato crops in the Andean region of South America. In most potato cultivars, primary and secondary symptoms are mild or severe mottles . Sensitive cultivars may react with initial top necrosis, leaf deformation and/or stunting . Plants growing under cool conditions may develop yellow spotting, blotching or more generalized yellowing on leaves.

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Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

The 42-kDa coat protein of Andean potato mottle virus acts as a transcriptional activator in yeast

BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH

Authors: Vidal, MS; Margis, R

Abstract

Interactions of viral proteins play an important role in the virus life cycle, especially in capsid assembly. Andean potato mottle comovirus (APMoV) is a plant RNA virus with a virion formed by two coat proteins (CP42 and CP22). Both APMoV coat protein open reading frames were cloned into pGBT9 and pGAD10, two-hybfid system vectors. HF7c yeast cells transformed with the p9CP42 construct grew on yeast dropout selection media lacking tryptophan and histidine. Clones also exhibited B-galactosidase activity in both qualitative and quantitative assays. These results suggest that CP42 protein contains an amino acid motif able to activate transcription of His3 and lacZ reporter genes in Saccharomyces cerevisiae. Several deletions of the CP42 gene were cloned into the pGBT9 vector to locate the region involved in this activation. CP42 constructions lacking 12 residues from the C-terminal region and another one with 267 residues deleted from the N-terminus are still able to activate transcription of reporter genes. However, transcription activation was not observed with construction pgCP42DeltaC57, which does not contain the last 57 amino acid residues. These results demonstrate that a transcription activation domain is present at the C-terminus of CP42 between residues 267 and 374.

Coat protein RNAs-mediated protection against Andean potato mottle virus in transgenic tobacco

PLANT SCIENCE

Authors: Neves-Borges, AC; Collares, WM; Pontes, JA; Breyne, P; Farinelli, L; de Oliveira, DE

Abstract

The expression of translatable sequences of either one of the two Andean potato mottle virus (APMoV) coat protein (CP) genes (CP22 and CP42) and of the nontranslatable sequence of CP42 in transgenic tobacco provided protection against APMoV. Resistance was mediated by CP transgene RNAs rather than the protein, as an inverse correlation between resistance and the accumulation levels of CPs transgene mRNAs was observed. These data indicated that a post-transcriptional gene silencing (PTGS) mechanism is likely involved in the APMoV CP RNA-mediated protection. Moreover, the HindIII-AccI restriction pattern of the CP22 transgene was different in susceptible and resistant transgenic plants, suggesting the involvement of methylation in PTGS. Southern blot experiments also revealed that CPs transgene insertion loci and organisation in the plant genome may play a role in determining the degree of protection. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.