Species Reactivity
Human, Mouse, Rat
Detection Method
Indirect ELISA
Intended Use
The ALX3 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ALX3 protein expression profile in cells. The kit can be used for measuring the relative amounts of ALX3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ALX3.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 1 plate
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-ALX3 Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
7. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
8. HRP-Conjugated Anti-Rabbit IgG Antibody: 6 mL (1x), Glass
9. HRP-Conjugated Anti-Mouse IgG Antibody: 6 mL (1x), Glass
10. Primary Antibody Diluent: 12 mL (1x), Clear
11. Ready-to-Use Substrate: 12 mL (1x), Brown
12. Stop Solution: 12 mL (1x), Clear
13. Crystal Violet Solution: 6 mL (1x), Glass
14. SDS Solution: 24 mL (1x), Clear
15. Adhesive Plate Seals: 4 seals
Citations
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Matsumoto, T; Yamada, A; et al. BMP-2 Induced Expression of Alx3 That Is a Positive Regulator of Osteoblast Differentiation. PLOS ONE 8:-(2013).
Zou, HZ; Harrington, JJ; et al. Highly methylated genes in colorectal neoplasia: Implications for screening. CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION 16:2686-2696(2007).