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Product Name
Cat.No.
Content
1 mg of lyophilized ODN 2007 Vaccine grade adjuvant
10 ml sterile endotoxin-free physiological water (NaCl 0.9%)
Storage and Stability
ODN 2007 is shipped at room temperature and should be stored at -20°C. Lyophilized product is stable 1 year when properly stored.
- Upon resuspension, prepare aliquots of ODN 2007 and store at -20°C. Resuspended product is stable 6 months when properly stored. Avoid repeated freeze-thaw cycles.
ODN 2007 Sequence
5'- tcg tcg ttg tcg ttt tgt cgt t -3' (22 mer)
Note: Bases in capital letters are phosphodiester and those in lower case are phosphorothioate.
Description
Synthetic oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs), such as ODN 2007, have been extensively studied as adjuvants. These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. CpG ODNs are recognized by TLR9, which is expressed exclusively on human B cells and plasmacytoid dendritic cells (pDCs), thereby inducing Th1-dominated immune responses. Pre-clinical studies, conducted in rodents and non-human primates, and human clinical trials have demonstrated that CpG ODNs can significantly improve vaccine-specific antibody responses.
Three types of stimulatory CpG ODNs have been identified, types A, B and C, which differ in their immune-stimulatory activities. Furthermore, CpG ODNs activate TLR9 in a species-specific manner. ODN 2007 is a type B CpG ODN specific for bovine/porcine TLR9. Type B CpG ODNs contain a full phosphorothioate backbone with one or more CpG dinucleotides and can strongly activate B cells.
Preparation of Sterile Stock Solution (2 mg/ml)
Add 500 μl endotoxin-free physiological water to the 1 mg ODN 2007 vial to obtain a solution at 2 mg/ml.
- Mix the solution by pipetting up and down.
Working Concentration
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