New Anti M13 Bacteriophage VHH Antibody

M13 is a filamentous bacteriophage and contains a single-stranded DNA genome (ssDNA) with a length of 6407 bp comprising nine genes that encode 11 proteins. Among 11 different proteins, five of them are coat proteins (proteins located on the capsid), including ~ 2700 copies of the major coat protein pVIII located on the side wall of the phage body, and five copies each of the pIII, pIV, pVII, and pIX minor coat proteins located on two ends (pIII and pVI on one end, pVII and pIX on the other end). pVIII (g8p) and pIII (g3p) are the most commonly exploited and modified coat protein for peptide display due to wide range of compatibility. With regard to interacting with foreign molecules, the pIII proteins are primarily responsible for biorecognition, while pVIII proteins are involved in the structural assembly of phage.

Fig. 1 Sensitivity of Anti-M13 Mab (1:10000 dilution)

The replicative form of M13 is circular double-stranded DNA similar to plasmids that are used for many recombinant DNA processes, and the virus has also been used for phage display, directed evolution, nanostructures and nanotechnology applications.

Creative Diagnostics develops a series of M13 antibodies which are VHH domain of alpaca IgG2b/2c fused to Rabbit Fc(mutation). Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated.

Performance

Fig. 2 Sensitivity of Anti-M13 Mab (1:10000 dilution)