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Creative Diagnostics has designed Magic Fast™ Antibody SN38 Conjugation Kit for antibody (IgG) labeling of SN38, the active metabolite of irinotecan, for early stage screening of your antibody.
Irinotecan is on the World Health Organization's List of Essential Medicines (the most effective and safe medicines needed in a health system). SN38 is 1000-times more active than irinotecan, but is not generally used as a drug due to its poor water solubility. If conjugated to a protein or antibody, the protein/antibody can transport SN38 to the active site. This kit includes O-succinyl SN38 NHS ester, which can be coupled to the antibody directly via surface amines in a single step. The product is then purified to remove any unreacted drug.
| Cat. No. | Antibody Amount per Reaction | Number of Reactions in Kit |
| CD4007-1mg | 1 mg | 1 |
| CD4007-3mg | 1 mg | 3 |
This kit provides materials to conjugate 1 mg of single antibody sample (IgG) with SN38 exposure to the chemotherapeutic drug.
| Kit Components | Name | Quantity (CD4007-1mg) | Quantity (CD4007-3mg) | Storage condition |
| Set 1 | O-succinyl SN38 NHS ester | 1 unit | 3 unit | -20°C (Keep dry) |
| Set 2 | Solution A | 0.5 mL | 1.5 mL | 2-8°C |
| Buffer A | 4 mL | 12 mL | ||
| Storage Buffer (1 x PBS) | 20 mL | 60 mL | ||
| ADC Stabilizing PBS Buffer (5x) | 0.5mL | 1.5 mL | ||
| Centrifugal Filter Devices | 2 | 6 | ||
| Desalting Column | 1 | 3 | ||
| Collection Tubes | 4 | 12 | ||
| * Note: The kit above is designed for IgG antibodies and proteins. | ||||
Fig. 1. Conjugation Chemistry
Fig. 2. Schematic diagram of the workflow for preparing antibody-SN38 conjugates starting with 3 mg of IgG (volume of reagents varies if the amount of IgG is < 3 mg).
| Cat No. | Product name | |
| CD4007-1 mg | Magic Fast™ SN38 Conjugation Kit (1 mg) | Inquiry |
| CD4007-3 mg | Magic Fast™ SN38 Conjugation Kit (3 mg) | Inquiry |
| Note: The kit above is designed for IgG antibodies and proteins. | ||
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