Creative Diagnostics has designed this Magic Fast™ MMAE Conjugation Kit to label any IgG antibody with monomethyl auristatin E (MMAE) using a valine-citruline p-aminobenzylcarbamate (VC-PAB) linker for early-stage screening of your antibody.
The kit includes maleimide-activated VC-PAB-MMAE, which can be coupled directly to the IgG following reduction and alkylation in a single step. The product is then purified to remove any unreacted drug.
| Cat. No. | Antibody Amount per Reaction | Number of Reactions in Kit |
| CD4006-100μg | 100 μg | 1 |
| CD4006-300μg | 100 μg | 3 |
| CD4006-1mg | 1 mg | 1 |
| CD4006-3mg | 1 mg | 3 |
This kit provides materials to conjugate single antibody sample (IgG) with MMAE using VC-PAB linker. The protocol is optimized for IgG with a molecular weight of 150 KDa to achieve an average of 4 drugs per antibody (DAR = 4). For other antibodies, the DAR may vary.
| Kit component | Name | Column condition |
| Set 1 | MC-VC-PAB-MMAE | -20°C |
| Reagent A | ||
| Set 2 | Solution A | 2-8°C |
| Reducing Buffer | ||
| Labeling Buffer | ||
| Storage Buffer (1 x PBS) | ||
| Centrifugal Filter Devices | ||
| Collection Tubes for Filter | ||
| Desalting Spin Column | ||
| Collection Tubes for Spin Column | ||
| 0.5 mL Eppendorf Tube(s) | ||
| 1.5 mL Centrifuge Tubes | ||
| Hazardous Waste Bag(s) | ||
| * Note: The kit above is designed for IgG antibodies and proteins. | ||
Fig. 1. Conjugation Chemistry
Fig. 2. Schematic workflow diagram for preparing antibody-MMAE conjugates.
| Cat No. | Product name | |
| CD4006-100μg | Magic Fast™ MMAE Conjugation Kit (With VC-PAB Linkage) (100 μg) | Inquiry |
| CD4006-300μg | Magic Fast™ MMAE Conjugation Kit (With VC-PAB Linkage) (300 μg) | Inquiry |
| CD4006-1 mg | Magic Fast™ MMAE Conjugation Kit (With VC-PAB Linkage) (1 mg) | Inquiry |
| CD4006-3 mg | Magic Fast™ MMAE Conjugation Kit (With VC-PAB Linkage) (3 mg) | Inquiry |
| Note: The kit above is designed for IgG antibodies and proteins. | ||
