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Liraglutide is a human glucagon like peptide-1 (GLP-1) receptor agonist. It simulates the action of endogenous GLP-1 in the human body, promotes glucose dependent insulin secretion, delays gastric emptying, and inhibits glucagon secretion. These effects together help regulate blood sugar levels, making it an important drug for the treatment of type 2 diabetes and obesity.
The uniqueness of Liraglutide lies in its acylation modification on the structure, which enables it to bind with albumin, thereby prolonging its half-life in vivo and achieving the convenience of once daily administration. Accurately determining the concentration of liraglutide in biological samples is crucial in pharmacokinetics, pharmacodynamics, and clinical research. This is exactly the area where our high-sensitivity ELISA detection kit excels.
Figure 1. Liraglutide Injection.
The Liraglutide High Sensitivity ELISA Kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) format, widely regarded as the gold standard for quantitative protein detection. This method utilizes two highly specific antibodies that recognize distinct epitopes on the liraglutide molecule, ensuring exceptional specificity and minimizing cross-reactivity with other GLP-1 analogs or endogenous peptides.
Figure 2. Liraglutide.
The assay workflow begins with pre-coated capture antibodies immobilized on the surface of 96-well microplate wells. When samples or standards are added, liraglutide molecules in the sample bind specifically to these capture antibodies. After a washing step to remove unbound components, a biotinylated detection antibody is introduced, forming a sandwich complex with the captured liraglutide. Streptavidin-horseradish peroxidase (HRP) conjugate is then added, which binds to the biotinylated detection antibody, creating an enzyme-labeled complex .
The final step involves adding a chromogenic substrate that undergoes enzymatic conversion by HRP, producing a colored reaction product. The intensity of the color is directly proportional to the concentration of liraglutide in the sample, measured spectrophotometrically at a specific wavelength (typically 450 nm with a reference wavelength of 630 nm).Sample Compatibility
Sensitivity Range
Kit Specifications
Our Liraglutide high-sensitivity ELISA detection kit undergoes strict quality control to ensure excellent performance.
Kit Composition
| Parameter | Details |
| Product name | Liraglutide High Sensitivity ELISA Kit |
| Intended Use | The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of liraglutide in serum, plasma. |
| Storage | After you receive the kit, all the components should be stored in the refrigerator (4-8°C) also up to 1 year. Long term storage, improper storage conditions and large temperature fluctuation cycles may cause precipitates in the TMB solution. These precipitates should not affect the assay noticeably. Nevertheless, if you observe such precipitates, we recommend to avoid them by allowing them to sink to the bottom. |
| General Description | Liraglutide is an acylated glucagon-like peptide-1 (GLP-1) agonist, derived from human GLP-1-(7-37), a less common form of endogenous GLP-1. It reduces meal related hyperglycemia, delaying gastric emptying, and suppressing prandial glucagon secretion. Liraglutide is sold as a medication used to treat diabetes mellitus type 2 and obesity. The prolonged action of liraglutide (as compared to endogenous GLP-1) is achieved by attaching a fatty acid molecule at one position of the GLP-1-(7-37) molecule, enabling it to both self-associate and bind to albumin within the subcutaneous tissue and bloodstream. The active GLP-1 is then released from albumin at a slow, consistent rate. |
| Standard Curve |  |
Reproducibility and Consistency
Reputable ELISA kits are manufactured under strict quality control standards. The inclusion of a comprehensive set of standards and controls within the kit allows researchers to generate a reliable standard curve for each assay.
High Specificity for Liraglutide
A well-designed high-sensitivity ELISA kit uses highly specific antibodies that are engineered to bind exclusively to Liraglutide. This minimizes the risk of cross-reactivity with other related molecules, such as endogenous GLP-1 or other GLP-1 receptor agonists.
Cost Effectiveness
ELISA kits are less expensive than RT-PCR, especially for high-throughput detection. It requires minimal equipment (microplate readers) and can be performed by trained technicians.
Low absorbance may result from improper reagent storage, expired reagents, insufficient incubation times, incorrect temperature during incubations, or inadequate washing steps. Ensure all reagents are within their expiration dates, properly stored, and equilibrated to room temperature before use. Verify incubation times and temperatures according to the protocol.
Poor linearity can result from improper standard dilution, pipetting errors, or contamination of standards. Prepare fresh standard dilutions carefully using precision pipettes, ensuring thorough mixing at each step. Avoid touching the tip to the solution in the receiving well when serially diluting.
While the kit is validated for serum, plasma, and cell supernatants, other sample types (such as cerebrospinal fluid or urine) may require validation. We recommend performing spike-and-recovery experiments to assess matrix effects before analyzing new sample types.
Therapeutic liraglutide levels are associated with improved glycemic control, reduced HbA1c levels, and weight loss in clinical studies. Monitoring liraglutide concentrations can help explain variability in patient response and guide dosage adjustments, particularly in complex cases with comorbidities like cardiovascular disease or chronic kidney disease.
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