Knockout Cell Lines - Creative Diagnostics

What is CRISPR/Cas9?

CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) is a widely used technology that uses sgRNA to guide Cas endonuclease to perform specific DNA modifications on targeted genes. The CRISPR/Cas9 system is widely present in prokaryotic genes and is an adaptive immune defense mechanism evolved by bacteria and archaea in response to constant attacks by viruses and plasmids.

The action process of CRISPR/Cas9 is mainly divided into two stages. The first stage is to capture exogenously introduced sequences. For the commonly used CRISPR/Cas9 system, the first stage is the binding of sgRNA and Cas9 protein. In the second stage, the transcribed crRNA forms pre-crRNA, which guides the Cas9 protein to cut the same target region on the genome as the sgRNA, thereby achieving the purpose of gene editing.

Gene Editing Services

Understanding the unique requirements of researchers, Creative Diagnostics offers customizable knockout cell lines. Researchers can request specific genes to be knocked out or choose from a selection of pre-designed knockout cell lines. Our experienced scientists and technical experts provide comprehensive technical support to researchers from guidance in selecting the appropriate knockout cell lines to troubleshooting experimental protocols.

Advantages of our CRISPR knockout service include:

High editing efficiency, simple construction, and easy operation

Enables cost-effective and flexible gene knockout

Broad spectrum, no gene, cell, or species restrictions

Enables simultaneous gene targeting of multiple target sites

Rich in functions, including knocking out, inserting, inhibiting, and activating target genes

High-Quality Knockout Cell Lines from Creative Diagnostics

Creative Diagnostics boasts an extensive gene catalog, encompassing a wide range of species, including humans, mice, rats, and more. This comprehensive collection enables researchers to choose from a diverse pool of knockout cell lines tailored to their specific research needs. We have constructed various knockout cell lines, such as:

Cell Type	Typical Cell Lines	Quality Control
Tumor Immune Cells	Jurkat, HepG2, SK-MES-1, etc.	PCR + Sanger sequencing, etc.
Induced Pluripotent Stem cells	iPSC	PCR + Sanger sequencing + immunofluorescence, etc.
Stem Cell	H1, H9	PCR + Sanger sequencing + immunofluorescence, etc.

The advantages of our knockout cell lines include the following:

Easy to use: Our knockout cell lines are designed for easy integration into various experimental setups, allowing researchers to save time and resources.

Reliable results: We provide knockout cell lines with well-characterized genotypes and phenotypes. Controls are supplied in all orders to ensure reliable and repeatable results.

Quality control: Our knockout cell lines undergo rigorous quality control checks, including genomic and proteomic validation, to ensure their authenticity and functionality.

The Importance of Knockout Cell Lines in Scientific Research

Unraveling gene function
Knockout cell lines play a vital role in unraveling the complex functional relationships between genes and their corresponding proteins. By selectively disabling a specific gene, researchers can observe the resulting changes in cellular behavior and identify the gene's contribution to various biological processes.

Disease modeling and drug discovery
Knockout cell lines are invaluable in the study of human diseases. By creating cell lines that mimic specific genetic mutations associated with diseases, researchers can gain crucial insights into disease mechanisms and develop potential therapeutic interventions. These cell lines serve as invaluable tools for drug discovery and preclinical testing.

Pathway analysis
Another significant application of knockout cell lines is pathway analysis. By systematically disrupting genes involved in a particular pathway, researchers can decipher the intricate network of interactions and signaling cascades that govern cellular processes. This knowledge is crucial for understanding disease progression and identifying potential targets for therapeutic intervention.

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