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The Human Urotensin II (UII) ELISA Kit is a high-performance, ready-to-use diagnostic tool designed for the precise and specific quantification of Human Urotensin II (UII) in biological samples (e.g., serum, plasma, cell culture supernatants). As a key regulatory peptide in cardiovascular homeostasis and metabolic regulation, UII plays critical roles in vascular tone modulation, cardiac function, and metabolic disorder progression (e.g., hypertension, obesity-related complications). This kit leverages the enzyme-linked immunosorbent assay (ELISA) technology to deliver reliable, reproducible results, enabling researchers to explore the molecular mechanisms of UII in cardiovascular and metabolic diseases, and support drug discovery and biomarker validation studies.
Urotensin II (UII) is a potent vasoactive cyclic peptide that plays a significant role in the cardiovascular system, acting as a powerful vasoconstrictor. It is involved in various physiological and pathophysiological processes, including the regulation of blood pressure, cardiac function, and glucose metabolism. Accurate measurement of UII levels is essential for understanding its role in conditions such as hypertension, heart failure, atherosclerosis, and metabolic syndrome.
Figure 1. Vasoactive peptide.
This ELISA kit employs a sandwich immunoassay principle. The microplate is pre-coated with an antibody specific to Human Urotensin II. During the assay, UII antigens in samples or standards bind to these immobilized antibodies. Following a series of incubations and washes, which include the addition of a biotinylated detection antibody and enzyme-conjugated streptavidin, a colorimetric reaction occurs . The intensity of the color produced is directly proportional to the concentration of Urotensin II present in the sample , allowing for accurate quantification.
Sample Compatibility
Sensitivity Range
Main Uses
Feature/Specification
Performance
Key Features of the Product
Intended Use
| Parameter | Details |
| Product name | Human Urotensin II ELISA Kit |
| Sample | plasma |
| Intended Use | This Enzyme Immunoassay kit is designed to detect a specific peptide and its related peptides based on the principle of "competitive" enzyme immunoassay. |
| Storage | Store the unopened kit at 4°C upon receipt and when it is not in use. For more detailed information, please download the following document on our website. |
The successful development of the Human Urotensin II ELISA Kit relies on the use of high affinity and high specificity paired antibodies, which target different epitopes of Urotensin II molecules and form the basis for sandwich detection. This design greatly reduces the interference of non-specific substances in the sample matrix, ensuring accurate specific binding and signal detection even in complex serum or plasma samples. The detection linear range of the reagent kit is wide, which allows for accurate detection of low-level UII under physiological conditions and abnormally high concentrations under pathological conditions without the need for tedious pre dilution or concentration steps on the sample. Excellent intra - and inter batch precision (CV<10%) ensures high reproducibility of experimental data, enabling stable and consistent results for both long-term longitudinal studies and large-scale clinical sample screening, providing solid data support for high-quality scientific research achievements.
Time and Cost Efficiency
The 3-hour total assay time is 30–50% faster than traditional radioimmunoassays (RIAs) or Western blotting. Additionally, the ready-to-use components (pre-coated plates, pre-diluted conjugates) eliminate time-consuming reagent preparation, reducing labor costs and experimental errors.
Robust Matrix Tolerance
Unlike kits prone to matrix interference, this kit includes a specialized sample dilution buffer that neutralizes matrix effects. Validation with diverse sample types (e.g., hyperlipidemic serum, EDTA-plasma) ensures accurate results across research cohorts.
Unmatched Precision
Compared to other UII detection kits, this ELISA kit achieves lower intra-assay and inter-assay CVs, minimizing experimental variability. This precision is critical for longitudinal studies or small-magnitude changes.
The Human Urotensin II (UII) ELISA Kit is an indispensable tool for cardiovascular and metabolic research, offering precise, sensitive, and user-friendly quantification of human UII. By overcoming the limitations of traditional detection methods, it empowers researchers to unravel the complex roles of UII in disease pathogenesis, validate potential biomarkers, and accelerate the development of targeted therapies for cardiovascular and metabolic disorders. Its consistent performance, broad applicability, and efficiency make it a valuable asset for both basic and translational research laboratories.
A1: No. This kit is specifically designed for human UII, using monoclonal antibodies that recognize the unique amino acid sequence of human UII. Cross-reactivity with UII from rodents (rat/mouse) or other species is negligible. For non-human UII detection, please select species-specific UII ELISA kits (e.g., Rat UII ELISA Kit, Mouse UII ELISA Kit).
A2: To preserve UII stability:
Cell culture supernatants: Collect and centrifuge at 1000×g for 5 minutes to remove cells, then store at -80°C immediately.
Avoid repeated freeze-thaw cycles (limit to ≤3 times), as this may cause UII degradation. Before testing, thaw samples at room temperature, mix gently, and centrifuge again if precipitates form.
A3: This kit operates on a sandwich ELISA principle. Essentially, a capture antibody specific to Urotensin II is immobilized on the plate. The sample is added, and UII binds to this antibody. A biotinylated detection antibody is then added, followed by an enzyme-streptavidin conjugate. A substrate solution is added, producing a color signal proportional to the amount of UII present.
A4: Yes, Creative Diagnostics provides technical support for its products. You can contact their technical team with questions regarding the assay protocol or troubleshooting.
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