The Human Erythropoietin (EPO) ELISA Kit is a robust and sensitive in vitro diagnostic tool designed for the accurate quantification of human Erythropoietin in various biological samples, including serum, plasma, and cell culture supernatants. Utilizing a sophisticated sandwich enzyme-linked immunosorbent assay (ELISA) format, this kit provides researchers and clinicians with a reliable method to obtain precise data crucial for studying erythropoiesis, diagnosing hematological disorders, and evaluating renal function. Its optimized protocol ensures high specificity and reproducibility, making it an indispensable asset for clinical laboratories and biomedical research.
Erythropoietin (EPO) is a glycoprotein with a molecular weight of approximately 35000 to 40000. It stimulates the proliferation and differentiation of bone marrow erythroid progenitor cells to generate red blood cells, making it a key drug for treating chronic renal anemia. The recombinant form of rhEPO was successfully developed in 1984 and later expanded for use in anemia disorders such as multiple myeloma and myelodysplastic disorders. RhEPO promotes erythropoiesis by activating erythroid specific genes and delaying cell apoptosis. Its receptor is also expressed in non-hematopoietic tissues and may be involved in neuronal protection and embryonic development regulation. Clinical application requires individualized administration, with an initial dose of 50-100U/kg, administered subcutaneously or intravenously three times a week. During treatment, blood pressure, hemoglobin levels, and iron supplementation should be monitored. Contraindications include uncontrolled hypertension, leukemia, and allergic constitution. Common adverse reactions include elevated blood pressure, itching, etc. Research has shown that rhEPO can reduce the need for blood transfusions and the incidence of anemia in premature infants.
Figure 1. Erythropoietin (EPO).
The ELISA (Enzyme-Linked Immunosorbent Assay) technology, the basis of this kit, leverages the specific antigen-antibody reaction. It uses a monoclonal antibody against human EPO coated on a microplate as the capture antibody, and a horseradish peroxidase (HRP)-conjugated polyclonal antibody as the detection antibody. After adding the sample and substrate, the intensity of the color reaction (measured by absorbance) is proportional to the EPO concentration in the sample, enabling accurate quantitative analysis. This kit addresses the need for rapid, high-throughput EPO detection in research, filling the gap between traditional low-sensitivity methods (e.g., radioimmunoassay with radioactive risks) and complex techniques (e.g., Western blot unsuitable for large-scale quantification).
Sample Compatibility
Sensitivity Range
Main Uses
Product Specifications
Intended Use & Research Applications
Key Features of the Product
| Parameter | Details |
| Product name | Human Erythropoietin, EPO ELISA Kit |
| Sample | serum, plasma, other biological fluids |
| Intended Use | This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). It is developed for quantitative measurement of Human EPO in serum, plasma and other biological fluids. |
| Storage | Store the kit reagents at 2-8°C. For more detailed information, please download the following document on our website. |
Compared with Western Blot, Western Blot is mainly used for qualitative/quantitative analysis of semi quantitative EPO detection, which requires a large sample size (~200 μ L) and a long experimental time (>8 hours). In contrast, this ELISA kit can perform quantitative detection with minimal sample consumption within<3 hours, making it more suitable for large-scale quantitative research.
Compared with Radioimmunoassay (RIA), RIA uses radioactive isotopes, which pose a radiation risk to operators and require special waste disposal. This ELISA kit uses non radioactive HRP substrate reaction to ensure safety and compliance with environmental regulations. In addition, the shelf life of RIA is relatively short (about 3 months), while the kit can be stored for 12 months.
Exceptional Sensitivity for Critical Low-Level Detection
This kit is engineered for high sensitivity, capable of accurately quantifying the low levels of progesterone present in the follicular phase of the menstrual cycle and in men. This is essential for obtaining clinically meaningful data across all physiological states.
Streamlined, Time-Efficient Workflow
The competitive ELISA format allows for a rapid and simplified procedure. With pre-coated plates and optimized reagents, the entire assay can be completed in approximately 90 minutes, facilitating timely decision-making in both research and clinical settings, and enabling high-throughput screening.
High Specificity for Accurate Clinical Assessment
The antibody used in this kit exhibits high affinity and specificity for progesterone, minimizing interference from other structurally similar steroid hormones. This ensures the accuracy and reliability of results, which is paramount for fertility assessment and pregnancy monitoring.
The Human EPO ELISA Kit is an indispensable tool for anemia and renal function research. With its high sensitivity, specificity, and user-friendly design, it enables accurate quantitative detection of human EPO in diverse samples, supporting studies on anemia pathogenesis, renal function evaluation, and drug development. Compared to alternative methods and competing products, it offers superior performance, versatility, and safety, while strict QC and comprehensive technical support ensure reliable results. Whether for academic research institutions, pharmaceutical companies, or clinical research centers, this kit provides a powerful solution to advance EPO-related research and contribute to the development of therapies for anemia and renal diseases.
A1: EPO measurement is crucial for the differential diagnosis of anemia, especially in distinguishing anemia of chronic disease from other types. It is also vital in assessing renal function, as the kidneys are the primary site of EPO production, and in investigating conditions like polycythemia vera.
A2: Serum or plasma samples should be collected using standard venipuncture techniques. For serum, allow blood to clot completely before centrifugation. For plasma, use appropriate anticoagulants like EDTA or heparin. Samples should be aliquoted and stored at -20°C or lower to prevent degradation and avoid repeated freeze-thaw cycles.
A3: The assay uses a sandwich ELISA principle. A capture antibody specific to human EPO is pre-coated onto the microplate. When the sample is added, EPO binds to this antibody. A biotinylated detection antibody is then added, forming an antibody-antigen "sandwich." This is followed by the addition of an enzyme-streptavidin conjugate and a colorimetric substrate. The intensity of the color produced is directly proportional to the amount of EPO present in the sample.
A4: No. This kit is specifically validated for the detection of human EPO due to the high specificity of the antibodies used. Cross-reactivity with EPO from other species is not guaranteed and would require validation by the user.
