Inquiry Basket
Loading ......
The Measles IgG ELISA Kit is a highly reliable and efficient in vitro diagnostic tool specifically developed for the quantitative detection of Measles virus IgG antibodies in human serum, plasma, and other relevant biological samples. This kit utilizes the enzyme - linked immunosorbent assay (ELISA) technique, which is widely recognized for its high sensitivity, specificity, and accuracy in immunological testing. It plays a crucial role in various fields such as clinical diagnosis, epidemiological surveillance, and vaccine efficacy evaluation.
Figure 1. Measles structure.
Measles is caused by the measles virus, which belongs to the Paramyxoviridae family and the Morbillivirus genus. It is an enveloped single stranded negative stranded RNA virus. The virus surface contains multiple key proteins, among which hemagglutinin (H protein) and fusion protein (F protein) are the key factors for its infection and pathogenicity. H protein is responsible for binding to receptors (such as CD150/SLAM and nectin-4) on the surface of host cells (such as lymphocytes and epithelial cells), while F protein mediates the fusion of viral envelope and cell membrane, allowing viral RNA to enter the cytoplasm for replication. These proteins are also the main targets for the human immune system to recognize and produce neutralizing antibodies.
Measles is one of the most contagious diseases known. The basic reproduction number is as high as 12-18, which means that a case can be transmitted to 12 to 18 people in a completely susceptible population.
Figure 2. Measles.
Measles is a highly contagious acute respiratory disease caused by the measles virus, mainly transmitted through droplets. Despite the existence of safe and effective vaccines, measles remains one of the leading causes of child mortality worldwide, especially in areas with low vaccination rates.The measles virus IgG antibody is a key indicator of adaptive immune response. After acute infection, IgM antibodies first appear, followed by the production and sustained presence of IgG antibodies, which typically provide lifelong immunity. The quantitative detection of measles IgG antibodies has multiple important significance:
Technology Platform
Sample Type Compatibility
Sensitivity Profile
Kit Features
Performance Characteristics
Sample Processing Requirements
Data Calculation
Precautions
Assessment of Post-Vaccination Immunity: The measles vaccine is highly effective, but not all individuals develop a protective immune response after vaccination. Furthermore, immunity can wane over time, especially in individuals who received a single dose. This kit allows for the quantitative assessment of antibody titers in vaccinated individuals. A titer above a certain threshold (e.g., 200 mIU/mL) is generally considered protective.
Confirmation of Past Measles Infection
In cases where a patient presents with symptoms suggestive of measles but a clinical diagnosis is uncertain, the presence of a high titer of measles IgG antibodies, especially when a paired sample shows a significant rise in titer, can confirm a past infection. It's important to note that IgM antibodies are typically used for acute infection diagnosis, while IgG is a marker of long-term immunity.
| Parameter | Details |
| Product name | Measles IgG ELISA Kit |
| Sample | Serum, Plasma and Cerebrospinal Fluid. |
| Intended Use | The Measles Virus IgG tests are quantitative and qualitative immunoassays for the detection of human antibodies in serum or plasma directed against Measles Virus. The Measles Virus IgG assay is recommended for determination of immune status and for detection of intrathecal synthesized IgG antibodies in cerebrospinal fluid. |
| Performance Characteristics | To evaluate the performance characteristics of the Measles Virus IgG, 195 serum samples from seronegative donors, patient samples, sera from children and unselected samples were examined in comparison to Measles virus IgG ELISA test system from two other manufacturer. Analyses have been performed according to the corresponding instructions for use. Borderline results were not included in the calculation of sensitivity and specificity. |
| Storage | Measles Virus, in common with Mumps virus, Parainfluenza Viruses and RS Virus belongs to the family of paramyxoviridae and has a worldwide distribution. According to WHO estimates, one million individuals die as a consequence of Measles Virus infections every year. A number of countries, along with the WHO, had set themselves the goal of eradicating measles by a program of vaccination. |
The Measles IgG ELISA Kit is an invaluable tool for both clinical diagnostics and public health surveillance. Its ability to provide a precise, quantitative measure of immunity enables healthcare providers and public health officials to make informed decisions regarding vaccination strategies, outbreak management, and individual patient care. By utilizing this advanced serological method, we can continue to advance our efforts toward the global eradication of measles.
Please refer to the instruction manual, which usually provides conversion formulas or conversion factors. If not, please contact technical support.
It is usually recommended to have an initial dilution ratio of 1:101 to minimize sample matrix effects and ensure that the OD values of the vast majority of samples fall within the linear range of the standard curve. For samples with extremely high titers (OD values exceeding the highest standard), they should be further diluted and retested.
Check if the calibration material is prepared incorrectly, if there are bubbles in the well, if the enzyme-linked immunosorbent assay (ELISA) is functioning properly, or if the incubation time/temperature is accurate. Suggest preparing calibration samples again and running the standard curve.
Loading ......
