CRISPR-Cas9 Antibodies

Overview

The CRISPR/Cas9 system has revolutionized genome engineering by enabling precise, efficient, and programmable modification of DNA sequences in living cells. Derived from the adaptive immune system of prokaryotes, CRISPR/Cas9 is widely used in gene knockout, knock-in, base editing and epigenetic modulation. With such broad applications, robust tools for monitoring and validating CRISPR activity at the protein level have become increasingly important, especially for confirming expression, localization, and functional integrity of Cas9 and its variants.

Fig. 1 Schematic diagram of the CRISPR–Cas 9 system molecular mechanism.
(Janik E.; et al. Int J Mol Sci. 2020)

Critical Functions of CRISPR/Cas9 Antibodies

Antibodies play a pivotal role in supporting CRISPR/Cas9 research via enabling the detection, tracking, and analysis of Cas9 proteins and their engineered derivatives across multiple stages of the gene editing workflow.

Detection of Cas9 protein expression: Antibodies specific to SpCas9 or SaCas9 are commonly used to assess expression levels by western blotting or flow cytometry.

Examining subcellular localization: Functional genome editing requires nuclear import of Cas9, and immunofluorescence assays using anti-Cas9 antibodies provide direct visualization of its intracellular distribution, particularly when fused with nuclear localization signals or other functional domains.

The validation of editing efficiency: Prolonged Cas9 expression is often correlated with increased off-target mutations. Antibody-based tracking helps define optimal time windows for transient expression.

Evaluating Cas9 binding specificity: When combined with ChIP and primers targeting both intended and non-target regions, ChIP-grade antibodies can help determine whether Cas9 is correctly localized to its target site guided by a specific sgRNA.

Creative Diagnostics now offers a specialized portfolio of high-quality antibodies for the detection and characterization of CRISPR/Cas9. Engineered to support advanced genome editing research, these antibodies are validated for key applications and provide reliable, high-performance tools for researchers working across genome engineering workflows.

Performance Data