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Allopregnanolone is a pivotal endogenous neuroactive steroid, renowned for its potent positive modulatory effects on the GABAA receptor. As a key mediator of inhibitory signaling in the central nervous system, ALLO plays a fundamental role in regulating mood, stress response, sleep, cognition, and neuroprotection. Dysregulation of ALLO levels has been implicated in a spectrum of neuropsychiatric disorders, including major depressive disorder (MDD), postpartum depression (PPD), anxiety, and PTSD. Our Allopregnanolone ELISA Kit is a state-of-the-art immunoassay designed to provide researchers with a highly sensitive, specific, and reliable tool for the precise quantification of ALLO in diverse biological matrices, empowering advancements in neuroscience, endocrinology, and psychopharmacology.
Neurosteroids like Allopregnanolone represent a critical intersection between the endocrine and nervous systems. Synthesized de novo in the brain or derived from peripheral steroid precursors, ALLO rapidly modulates neuronal excitability. Its significance is highlighted by the recent approval of a synthetic analog (brexanolone) for treating PPD, underscoring the therapeutic relevance of this pathway. Accurate measurement of ALLO is therefore essential for understanding its physiological roles and pathological contributions. Our ELISA kit utilizes a robust competitive enzyme immunoassay format, offering a streamlined and accessible alternative to complex chromatographic methods like GC-MS, without compromising on data quality.
Figure 1. Allopregnanolone.
Sample Compatibility
Sensitivity Range
Main Uses
General Specifications
Performance
Intended Use
Key Features of the Product
| Parameter | Details |
| Product name | Allopregnanolone ELISA Kit |
| Sample | serum, plasma, fecal, urine, TCM |
| Intended Use | The Allopregnanolone Immunoassay kit is designed to quantitatively measure Allopregnanolone present in extracted serum, plasma, or dried fecal samples, or in diluted urine, and tissue culture media samples. |
| Storage | Store the kit reagents at 4°C. For more detailed information, please download the following document on our website. |
Disease-Related Research
Psychiatric disorders: PPD, anxiety, major depressive disorder (MDD), bipolar disorder (measuring allopregnanolone in CSF/serum)
Neurological disorders: AD, Parkinson's disease, epilepsy, traumatic brain injury (TBI) (quantifying levels in brain tissue/CSF)
Substance use disorders: Alcohol/opioid dependence (assessing allopregnanolone's role in withdrawal symptoms)
Pharmacological & Toxicological Research
Evaluating efficacy of neurosteroid-modulating drugs (e.g., allopregnanolone analogs for PPD, 5α-reductase inhibitors)
Testing environmental toxins/endocrine disruptors (e.g., bisphenol A) on allopregnanolone synthesis
Preclinical studies: Validating animal models of neurosteroid dysregulation (e.g., PPD mouse models)
Direct Insight into Neuroactive Pathways
This kit provides a direct measure of the biologically active GABAA modulator, ALLO, offering more relevant functional data than measuring its precursor, progesterone.
High-Throughput Efficiency
The 96-well microplate format allows for the simultaneous analysis of up to 80 samples (in duplicate, including standards), dramatically increasing lab productivity compared to low-throughput techniques.
Robust and Publisheable Data
The combination of high specificity and reproducibility minimizes variability, yielding clean, statistically powerful data suitable for high-impact publications and grant applications.
In the rapidly evolving field of neurosteroid research, precise and reliable quantification is paramount. Our Allopregnanolone ELISA Kit offers an unparalleled combination of sensitivity, specificity, and ease of use, making it the ideal solution for researchers dedicated to elucidating the complex roles of ALLO in health and disease. Empower your investigations into the brain's intricate chemistry with a tool you can trust.
A1: For most serum and plasma samples, direct application is possible. However, for complex matrices like tissue homogenates or CSF with potentially low concentrations, extraction and concentration are highly recommended to improve sensitivity and specificity. Please refer to the detailed protocol for guidance.
A2: ELISA is excellent for high-throughput screening and is more accessible to most labs. While LC-MS/MS offers ultimate specificity and can multiplex, it requires expensive instrumentation and specialized expertise. Our ELISA kit provides a robust and highly reliable alternative for most research applications.
A3: The kit contains one 96-well plate. After accounting for the standard curve and controls, one kit typically allows for the analysis of up to 40 unknown samples in duplicate.
A4: You will require a microplate reader capable of measuring absorbance at 450 nm (with a reference wavelength of 620-650 nm), a precision pipette, a plate shaker, and a washer (manual or automated).
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