c-di-AMP

Content
1 mg of lyophilized c-di-AMP Vaccine grade adjuvant.
10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

Storage and Stability
c-di-AMP is shipped at room temperature and should be stored at -20°C. Lyophilized product is stable 1 year when properly stored.
Upon resuspension, prepare aliquots of c-di-AMP and store at -20°C. Resuspended product is stable 6 months when properly stored. Avoid repeated freeze-thaw cycles.

Description
Cyclic diadenylate monophosphate (c-di-AMP) is an intracellular signaling molecule produced by bacteria. Administration of c-di-AMP can induce a strong immune response in vitro and in vivo. It was recently found that this cyclic dinucleotide induces the production of cytokines, such as type I interferons, through the STING/TBK1/IRF3 pathway. Due to its immunostimulatory properties, this molecule has been investigated as a vaccine adjuvant. Mucosal delivery of c-di-AMP elicits a balanced Th1/Th2 profile and Th17 response2, which is crucial against intracellular pathogens. This adjuvant acts through the recruitment of monocytes and granulocytes, and the maturation of dendritic cells.

Preparation of Sterile Stock Solution (2 mg/ml)
Add 500 μl endotoxin-free physiological water to the 1 mg c-di-AMP vial to obtain a solution at 2 mg/ml.
Mix the solution by pipetting up and down.

Vaccine Grade