Flagellin FliC / Recombinant flagellin from S.typhimurium
50 μg of lyophilized Flagellin FliC Vaccine grade adjuvant
10 ml sterile endotoxin-free physiological water (NaCl 0.9%)
Storage and Stability
Flagellin FliC is shipped at room temperature and should be stored at -20°C. Lyophilized product is stable 1 year at -20°C when properly stored.
- Upon resuspension, prepare aliquots of Flagellin FliC and store at -20˚C. Resuspended product is stable 6 months at -20°C when properly stored. Avoid repeated freeze-thaw cycles.
Flagellin FliC is a recombinant flagellin protein encoded by the fliC gene from Salmonella typhimurium. Bacterial flagellin, a TLR5 ligand, is a potent T-cell antigen and has potential as a vaccine adjuvant. Unlike other TLR agonists, flagellin tends to produce mixed Th1 and Th2 responses rather than strongly Th1 responses. It has been demonstrated that flagellin can act as a potent adjuvant in flu vaccines. Furthermore, flagellin can also signal through the NLRC4 inflammasome, although it is not known whether this pathway contributes to the adjuvant activity of flagellin.
Flagellin from Salmonella typhimurium has been tested in animal models. Experimental results in mice demonstrated that an intraperitoneal injection of flagellin can induce cytokine production and boost intestinal innate immune defense.
Preparation of Stock Solution (100 µg/ml)
Open vial lid carefully to avoid any loss of product.
- Add 500 µl of the sterile endotoxin-free physiological water to the 50 µg vial.
- Vortex until complete solubilization.