Structure-Dependent Interactions

Three-dimensional structures adopted by DNA and RNA dictate the precise interactions between proteins and nucleic acids which form the basis of gene regulation. Structure-dependent interactions between molecular structures such as G-quadruplexes, triplex DNA, and RNA hairpins perform essential functions in biological processes such as gene replication and transcription while also influencing epigenetic modifications and contributing to disease development like cancer. Creative Diagnostics provides a full range of services that employ near-infrared microarray protein chip technology for advanced high-throughput and high-sensitivity analysis of essential structure-dependent interactions for drug discovery and functional genomics research.

Figure 1. An example of a G-tetrad structure and G-quadruplex
(Source: Chen Y, et al. 2012)

Our Application-Focused Services

• Precise Detection and Characterization of Structure-Dependent Interactions for Targeted Drug Development:
  1. Application: Our platform allows for the detailed analysis of protein binding to specific nucleic acid structures, such as G-quadruplexes, which are implicated in tumor development and are promising targets for anticancer drugs. Our services include G-quadruplex complex conformation analysis with NMR validation alongside protein-nucleic acid dynamic binding kinetics analysis using Surface Plasmon Resonance (SPR) technology as well as drug target screening with binding mode identification.
  2. Benefit: New drug targets should be discovered by examining unique nucleic acid structures and then the binding of prospective drug candidates to these targets must be characterized to create highly specific and effective therapeutic treatments.
  3. Example: Evaluate small molecules for their effectiveness in stabilizing G-quadruplex structures within oncogene promoters and assess their binding kinetics to refine drug candidate selection.
• High-Throughput Multi-Parameter Joint Detection for Comprehensive Regulatory Network Analysis:
  1. Application: Our technology enables the simultaneous detection of various components involved in structure-dependent interactions, including DNA/RNA binding proteins, transcription factors, and epigenetic modifying enzymes. This allows for a holistic understanding of complex regulatory pathways. We support multi-target analysis in single samples, such as investigating tumor-related pathway protein interaction networks.
  2. Benefit: Understanding how various regulatory molecules interact with distinct nucleic acid structures enables researchers to better comprehend disease mechanisms and discover treatments targeting multiple pathways.
  3. Example: Conduct simultaneous analysis of multiple transcription factors and epigenetic modifiers as they bind to a unique DNA structure found in cancer cells.
• Customized Solutions for Diverse Research Applications:
  1. Application: We provide flexible and tailored solutions to meet a wide range of research needs, offering full process support from in vitro assays (e.g., EMSA, ChIP) to in vivo analysis (e.g., CRISPR complex analysis). Our services also include data integration and bioinformatics simulation for epigenetic regulatory network modeling.
  2. Benefit: Our services provide specialized experimental designs together with detailed data analysis to explore specific research questions on structure-dependent protein-nucleic acid interactions within different biological systems.
  3. Example: Develop a microarray study focused on CRISPR-Cas complex interactions with genomic regions that create non-standard DNA structures and apply bioinformatics techniques to analyze the resulting effects on gene expression.

Key Advantages of Our Technology Platform

• Near-infrared Fluorescence Labeling: Offers high signal stability, low background interference, supports full automation, and is compatible with a wide pH range and complex samples.
• Functionalized Silica Gel Membrane: Provides broad-spectrum binding capability for DNA, RNA, and proteins, with high sensitivity.
• Laser Confocal System: Enables single-molecule level interaction analysis.

Typical Application Areas

• Drug Development: G-quadruplex stabilizer screening, telomerase inhibitors.
• Cancer Research: Understanding the role of non-canonical DNA structures in tumorigenesis.
• Gene Therapy: Analyzing the interaction of therapeutic molecules with specific nucleic acid structures.
• Basic Research: Investigating the fundamental principles of structure-dependent protein-nucleic acid interactions.

Reasons to Choose Us

1. Innovative Technology
2. Full-Process Quality Control System
3. Rich Cross-Domain Application Experience
4. Customized Solutions
5. High Cost-Performance Ratio
6. Continuous Technological Innovation

Our Protein-Nucleic Acid Interaction (Structure-Dependent) Detection and Analysis Service, leveraging the advanced near-infrared microarray protein chip technology platform, provides you with high-throughput, high-sensitivity solutions. From basic research to drug development, our services cover multiple key areas in life science research. Our clients gain access to cutting-edge technology support together with complete quality assurance and personalized application experiences through our tailored solutions.

Contact our expert team to discover how we will provide your project with the optimal solution. We plan to collaborate with academic researchers and biotechnology companies to push scientific discovery forward.

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