The 3D cell culture chip is an advanced biotechnology platform that provides a highly biomimetic environment for cell research. By constructing three-dimensional structures at the microscale, the chip more closely replicates physiological conditions, enabling cells to grow and interact in a more natural manner. Compared with traditional 2D culture systems, 3D cell culture chips can more accurately simulate cellular behaviors and responses in vivo, thereby providing a more reliable experimental platform for applications such as drug screening, disease research, and tissue engineering.