Mouse anti-medaka vitellogenin Monoclonal antibody, clone DL-2I22

Antibody Isotype

IgG1, κ

Clone

DL-2I22

Species Reactivity

Japanese medaka (Oryzias latipes); Atlantic cod (Gadus morhua); Rainbow trout (Oncorhynchus mykiss); carp (Cyprinus carpio); tilapia (Oreochromis niloticus)

Immunogen

Lipovitellin purified from eggs of female Japanese medaka (Oryzias latipes).

Conjugate

Application Notes

ELISA: 1:100 - 1:1000; Western blot: 1:50 - 1:500
The monoclonal antibody CK-1H11 works well in both ELISA and western blot for a number of species. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA and is therefore not recommended to use for coating.

General Notes

This product consists of affinity-purified mouse monoclonal antibodies against vitellogenin from Japanese medaka (Oryzias latipes).
The monoclonal antibody binds with high affinity to vitellogenin and lipovitellin from Japanese medaka (Oryzias latipes). The antibody also cross-reacts with vitellogenin from a variety of different species including Atlantic cod (Gadus morhua), rainbow trout (Oncorhynchus mykiss), carp (Cyprinus carpio) and tilapia (Oreochromis niloticus). The degree of cross-reactivity differs between species and with the methods employed.
The monoclonal antibody works well in both ELISA and western blot for a number of species.