Mouse anti-medaka vitellogenin Monoclonal antibody, clone DL-5C4

Antibody Isotype

IgG1, κ

Clone

DL-5C4

Species Reactivity

Japanese medaka (Oryzias latipes); Sheepshead minnow (Cyprinodon variegatus); killifish (Fundulus heteroclitus)

Immunogen

Lipovitellin purified from eggs of female Japanese medaka (Oryzias latipes).

Conjugate

Application Notes

ELISA: 1:100 - 1:1000; Western blot: 1:50 - 1:500
The monoclonal antibody CK-4B3 works well in both ELISA and western blot. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA and is therefore not recommended to use for coating.

General Notes

This product consists of affinity-purified mouse monoclonal antibodies against vitellogenin from Japanese medaka (Oryzias latipes).
The monoclonal antibody binds with high affinity to vitellogenin and lipovitellin from Japanese medaka (Oryzias latipes). The antibody also cross-reacts to some degree with vitellogenin from other fish species including sheephead minnow (Cyprinodon variegatus) and killifish (Fundulus heteroclitus). The degree of cross-reactivity differs between species and with the methods employed.
The monoclonal antibody works well in both ELISA and western blot.