Vitamin B12 (VB12) Rapid Test (DTS832L)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
50T
Sample
Serum, whole blood
Intended Use
Vitamin B12 Rapid Test is a rapid, one step test for the qualitative detection of Vitamin B12 in human serum or whole bloodn samples.
Storage
Store at 4-30°C, DO NOT FREEZE or use beyond the expiration date. The shelf life is 12 months.
Detection Limit
The detection limit of VB12 with this test is about 0.5 ng/mL (ppb)
General Description
Vitamin B12 is a water-soluble vitamin that is necessary for DNA synthesis, the metabolism of amino acids and fatty acids1 which are required for normal blood formation, cell synthesis, and neurological functions in the human body. A deficiency in vitamin B12, which can occur due to inadequate absorption or intake, can lead to neurological, psychiatric, and hematological disorders. Vitamin B12 deficiency is defined as a serum vitamin B12 level of less than 148 pmol/L. The primary dietary sources of vitamin B12 are meats, fish, shellfish, and dairy products and as such the deficiency has been reported to be the highest among populations with predominantly a vegetarian or vegan diet. In the US, the deficiency rate among vegetarians has been estimated at 60%. At present, the main technical methods used in the detection of vitamin B12 are HPLC, ELISA, etc., these require professional equipment and instruments, and should not be popularized. The immunocolloid gold chromatography technique is simple, fast, and specific, and can be used for the rapid detection of vitamin B12.

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References


A missense mutation in TCN2 is associated with decreased risk for Congenital Heart Defects and may increase cellular uptake of vitamin B12 via Megalin

ONCOTARGET

Authors: Li, Peiqiang; Huang, Lijuan; Zheng, Yufang; Pan, Xuedong; Peng, Rui; Jiang, Yueming; Finnell, Richard H.; Li, Haijie; Qiao, Bin; Wang, Hong-Yan

Deregulation of folate and vitamin B12 (VB12) metabolism contributes to the risk of congenital heart defects (CHDs). Transcobalamin (TCN2) is essential for transporting VB12 from blood to cells as TCN2-bound VB12 (holo-TC) is the only form for somatic cellular uptake. In this study, we performed an association study between common polymorphisms in 46 one carbon metabolism genes and CHD in 412 CHDs and 213 controls. Only two significant association signals in coding regions were identified: FTCD c. 1470C> T & TCN2 c. 230A> T. The only missense mutation, TCN2 c. 230A> T, was further validated in 412 CHDs and 1177 controls. TCN2 c. 230T is significantly associated with reduced CHD risk in North Chinese (odds ratio = 0.67, P = 4.62e-05), compared with the 230A allele. Interestingly, the mean level of plasma holo-TC in women with the TA genotype was 1.77-fold higher than that in women with the AA genotype. Further analysis suggested that c. 230A> T enhanced the cellular uptake of holo-TC via the LRP2 receptor. Our results determined that a functional polymorphism in TCN2 contributes to the prevalence of CHDs. TCN2 c. 230A> T is significantly associated with a reduced CHD risk, likely due to TCN2 c. 230T improving the interaction between holo-TC and its LRP2 receptor.

Synergistic Role of Microwave and Perturbation toward Synthesis of Hierarchical Porous MOFs with Tunable Porosity

INORGANIC CHEMISTRY

Authors: Laha, Subhajit; Chakraborty, Anindita; Maji, Tapas Kumar

Creating hierarchical porosity in MOFs has attracted significant interest due to their immense potential in a wide range of applications from materials to life science. Herein, we report a unique methodology of combining perturbation assisted nanofusion (PNF) with microwave (MW) stimuli to generate wide additional pores from (5-18) nm in the prototype MOF, NiMOF-74. An optimized combination of microwave exposure, perturbation in form of stirring, and solvent effect induces additional mesoscale porosity by fusion of MOF nanoparticles. The effect of microwave is realized by varying reaction time and medium using a range of solvents having different dielectric constant (DMSO, DMF, DMA, acetone, EA, and THF). Introducing this method, for the first time, we are able to generate a wide scale mesopore by fusion of nanoscale microporous MOF within a short reaction time by vigorous stirring, without using any template. This additional mesopore, thus generated, has been exploited by encapsulating 4.6 mu mol.g(-1) of large biomolecule Vitamin B-12 (VB12).

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