Vedolizumab drug level ELISA

Sample

Serum, Plasma (EDTA, Heparin)

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The Vedolizumab ELISA has been especially developed for the quantitative analysis of free vedolizumab in serum and plasma samples.

Storage

The kit is shipped at ambient temperature (10-30°C) and should be stored at 2-8°C for long term storage. Keep away from heat or direct sunlight. The strips of microtiter plate are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2-8°C.

Precision

Intra-assay and inter-assay CVs < 30%

Sensitivity

The lowest detectable level (Lowest detection limit, LOD) that can be distinguished from the zero standard is 1 ng/mL
Functional sensitivity (Limit of quantification-LOQ): 3 ng/mL

General Description

Vedolizumab binds to α4β7 integrin, a key mediator of gastrointestinal inflammation expressed on the surfaces of T and B lymphocytes. By selectively inhibiting the α4β7 integrin, vedolizumab inhibits adhesion of lymphocytes to its natural ligand, mucosal addressin cell adhesion molecule-1 (MAdCAM-1), thereby preventing lymphocytic cells from entering the gut lamina propria and gut-associated lymphoid tissue (GALT). Specifically inhibiting this pathway alleviates GI inflammation without impairing systemic immune responses.