Vaccinia Capping Enzyme ELISA Kit

Name: Vaccinia Capping Enzyme ELISA Kit
SKU: DEIA-BZ003
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Biological products.

Detection Method

sELISA

Intended Use

For the quantitative measurement of Vaccinia Capping Enzyme in biological products. It is applicable to theoptimization of purification process of biological products, impurity control of intermediate process and release testing of final products.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Standard, Lyophilized	2vials	2-8°C
2	Coated Plate	8wells×12 strips	2-8°C
3	Dilution Buffer 1	45mL×1vial	2-8°C
4	Dilution Buffer 2	30mL×1vial	2-8°C
5	Wash Buffer(20×)	50 mL×1vial	2-8°C
6	Detection Antibody (100×)	120μL×1tube	2-8°C
7	Streptavidin-HRP(500×)	40μL×1tube	2-8°C
8	TMB Substrate	15mL×1vial	2-8°C
9	Stop Solution	10mL×1vial	2-8°C
10	Sealing Film	4 pieces	2-8°C

Storage

This kit should be stored at 2-8°C before the kit opening. The kit has a shelf life of 12 months from the date of manufacture. After opening, store the kit at 2-8°C and use within 30 days

Detection Range

0.125 - 8 ng/mL

Sensitivity

Limit of quantification: 0.125 ng/mL

General Description

The cap structure is a modification at the 5' end of eukaryotic mRNA formed after transcription. Vaccinia capping enzyme is an effective catalyst in forming this cap structure. Therefore, for biological products utilizing vaccinia capping enzyme, residual levels must be quantitatively assessed.
This ELISA kit employs a double-antibody sandwich format to detect vaccinia capping enzyme residues. Standards and samples are added to microplate wells pre-coated with an anti-vaccinia capping enzyme antibody. A biotin-labeled detection antibody is added, followed by Streptavidin-HRp, forming an antibody-antigen-biotinylated antibody-Streptavidin-HRP complex.TMB substrate is then added for color development. TMB turns blue upon catalysis by HRP and transitions to yellow after the addition of stop solution. The intensity of the yellow color is directly proportional to the amount of capping enzyme present in the sample.

Standard Curve

Standard Curve Fitting: Plot standards' concentrations(x-axis) against OD450 values (y-axis). Apply 4-parameter logistic(4PL) regression for curve fitting.

The standard curve is obtained by 4-parameter fitting with the theoretical standard concentrations and the corresponding OD values (as shown in the figure below).