Ultrasensitive Immunoprecipitation Services

What is Immunoprecipitation (IP) Services?

IP is a technology that uses antibodies specific to the protein of interest to purify a given protein or protein complex from a mixture of other proteins and molecules contained in a biological sample. It can be used to detect and analyze protein expression, post-translational modifications and protein-protein interactions under native or denaturing conditions. Antibodies against a protein of interest are used to incubate with cell/tissue lysates bound to a solid support to capture the target protein and any proteins that are bound to it. Once non-specific proteins and other components are eluted, the target protein complex can be collected and subjected to further analysis through downstream applications. IP services are performed in molecular biology, biochemistry and proteomics to understand signaling pathways, validate biomarkers and dissect functional protein networks with high specificity, sensitivity and reproducibility.

Applications of Immunoprecipitation (IP) Services

Protein–protein interaction analysis

Post-translational modification detection (e.g., phosphorylation, ubiquitination)

Protein expression and abundance studies

Biomarker validation and target identification

Signal transduction pathway investigation

Protein complex characterization

Antibody validation and specificity testing

Chromatin-associated protein enrichment (in ChIP workflows)

Why Choose Creative Diagnostics' Immunoprecipitation (IP) Services?

Bio-Rad ChemiDoc™ MP

Thermo Scientific Orbitrap Fusion™ Lumos

Beckman Coulter Avanti J-26 XP

Creative Diagnostics offers a complete range of Immunoprecipitation (IP) Services to aid in the study of protein expression, interactions and modifications. Immunoprecipitation is a vital tool to understand the complex signaling pathways and functional proteomics of the cell. Creative Diagnostics has years of experience and specialized knowledge in protein biochemistry and offers you tailored Immunoprecipitation assay solutions to help you reach your varied research goals. Applications for IP services include identifying protein binding partners, post-translational modifications or to help characterize protein complexes. Our platform combines our ability to select high affinity antibodies along with optimized immunoprecipitation and sensitive detection methods to deliver consistent, high quality and reproducible results. We provide:

Highly specific antibody screening and validation services ensure accurate identification and enrichment of target proteins.

Optimized primary and denaturing immunoprecipitation protocols are suitable for a variety of research targets and protein types.

We process a wide range of sample sources, including cell lysates, tissue extracts, and recombinant protein samples.

Efficient protein complex capture technology supports in-depth investigation of protein-protein interactions.

Strict experimental process control, including precise control of binding, wash, and elution conditions, reduces nonspecific background.

An experimental platform compatible with various downstream analysis methods (such as Western blot, mass spectrometry, and ELISA).

Scalable high-throughput immunoprecipitation capabilities are suitable for large-scale proteomics screening projects.

Customized experimental design and technical consulting services meet our clients' diverse needs for mechanistic research and drug target validation.

Our Services

To meet the research needs of rapid and accurate analysis of proteins and their interactions, we provide comprehensive Immunoprecipitation (IP) Services. Relying on advanced cell lysis and immunoprecipitation technologies, we can efficiently capture target proteins or protein complexes under physiological conditions, helping to analyze protein functions and their regulatory mechanisms. Our services include optimized sample preparation processes, high-specificity antibody screening, strict immunoprecipitation protocols, and compatibility with a variety of downstream detection methods (such as Western blot, mass spectrometry analysis) to ensure high data quality and high resolution. This platform is widely used in protein-protein interaction studies, signaling pathway analysis, post-translational modification identification, and exploration of disease-related protein functions. With a strict quality control system and deep technical accumulation, we ensure that each project is highly specific, reproducible, and consistent. Our core services include:

Experimental Design and Technical Consulting

Provide customized project design, clarifying the target protein, research objectives, and expected outputs
Recommend the most suitable primary or denatured IP protocols based on protein characteristics
Provide sample preparation advice and guidance on antibody selection

Sample Pretreatment and Lysis

Process a variety of sample types (cells, tissues, recombinant proteins, etc.);
Preserve native protein-protein interactions under primary conditions;
Offer denaturing conditions to investigate stable binding or post-translational modifications.

Antibody Screening and Validation

Screen for high-affinity and high-specificity antibodies.
Can use customer-supplied antibodies or recommended commercial or custom antibodies.
Validate antibody binding in immunoprecipitation.

Immunoprecipitation Procedures

Use a variety of supports, including Protein A/G magnetic beads and agarose beads.
Control binding, wash, and elution conditions to minimize nonspecific binding.
Rigorously replicate experimental procedures to ensure reproducibility and consistency.

Downstream Detection and Analysis

Western Blot: Verify target proteins and their interacting proteins;
Silver/Coomassie Staining: Assess the purity of precipitated products;
Mass Spectrometry (MS): Proteomic analysis and complex identification;
ELISA or other quantitative methods for quantitative detection of specific proteins.

Data Collation and Result Reporting

Provide raw image data (e.g., Western blot images);
Provide detailed experimental records, protocol steps, and key reagent information;
If requested, include analysis results such as protein identification lists and interaction network diagrams.

Derivatization Services (Optional)

Co-IP (Co-immunoprecipitation): Study protein-protein interactions;
ChIP (Chromatin Immunoprecipitation): Explore protein-DNA interactions;
IP-MS Integrated Service: A one-stop service from immunoenrichment to mass spectrometry analysis;
Stable Cell Line Construction and Expression System Optimization: Assist in experiments with difficult-to-express proteins.

Immunoprecipitation (IP) Services Process

Prepare cell or tissue samples.

Lyze samples and extract proteins.

Capture target proteins and complexes using specific antibodies.

Remove impurities and elute purified proteins.

Perform assay analysis and provide a report of the results.

Case Study

Mass spectrometry analysis for the peptide composition of an RTK protein complex in IP

This mass spectrometry analysis shows the peptide composition of an RTK protein complex enriched using Creative Diagostics' immunoprecipitation (IP) service. The sample was derived from human cancer cells and immunoprecipitated with an anti-RTK antibody, followed by mass spectrometry identification. The major peaks in the figure (e.g., m/z = 1252.74, 1421.62, 1535.68, etc.) represent the peptides that are characteristic of RTKs and their known adaptors, e.g., Grb2 and Shc. This result verifies the success of enriching the RTK complex by immunoprecipitation and reliably identifying the major protein members by mass spectrometry and further discloses protein-protein interactions in cancer signaling pathways.

FAQs About Immunoprecipitation (IP) Services

How do I know if the antibody I choose is suitable for immunoprecipitation?

We'll help you evaluate the antibody's specificity and affinity to ensure it effectively captures your target protein and minimizes background noise.

Can immunoprecipitation detect weak protein-protein interactions?

Yes, but mild lysis conditions and optimized wash steps should be used to protect easily dissociated protein complexes.

Can I use immunoprecipitation to study post-translational modifications of proteins?

Absolutely! We support immunoprecipitation for a variety of modifications, including phosphorylation and ubiquitination.

If I only have a small amount of sample, can we still perform IP?

No problem. We have highly sensitive protocols optimized for low-input samples.

What other follow-up analyses can be performed after immunoprecipitation?

In addition to standard Western blots, we can also assist with mass spectrometry identification, ELISA quantification, and even proteomics studies.

Inquiry Basket

Loading ......

Inquiry