Trimethoprim ELISA Kit (DEIA-WZ037)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
Honey, Aquatic products and Milk
Intended Use
This ELISA kit is designed to detect TMP based on the principle of "indirect-competitive" enzyme immunoassay.
Storage
2-8°C
Precision
C.V. of the ELISA kit is less than 10%.
Detection Limit
Honey………………………………………..…1ppb
Aquatic products (fish and shrimp)………………….1ppb
Milk (raw milk and finished milk)…………………….1ppb
Sensitivity
Test Sensitivity: 0.02ppb
General Description
Trimethoprim (TMP) is a bacteriostatic antibiotic mainly used in the prophylaxis and treatment of urinary tract infections. TMP is commonly used with other sulfonamides to enhance the effect of them. Due to the potential side effect of its residue in food of animal origin, strict MRLs have been established.

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References


Design, synthesis and activity against Staphylococcus epidermidis of 5-chloro-2-or 5-chloro-4-methyl-9H-xanthen-9-one and some of its derivatives

CHEMICAL BIOLOGY & DRUG DESIGN

Authors: Mazur, Gabriela; Skiba-Kurek, Iwona; Karczewska, Elzbieta; Panczyk-Straszak, Katarzyna; Jaworska, Joanna; Waszkielewicz, Anna M.

Ten new xanthone derivatives have been designed and synthesized for their potential antibacterial activity. All compounds have been screened against Staphylococcus epidermidis strains ATCC 12228 and clinical K/12/8915. The highest antibacterial activity was observed for compound 3: 5-chloro-2-((4-(2-hydroxyethyl)piperazin-1-yl)methyl)-9H-xanthen-9-one dihydrochloride, exhibiting MIC of 0.8 mu g/ml against ATCC 12228 strain, compared to linezolid (0.8 mu g/ml), ciprofloxacin (0.2 mu g/ml) or trimethoprim and sulfamethoxazole (0.8 mu g/ml). For the most active compound 3, genotoxicity assay with use of Salmonella enterica serovar Typhimurium revealed safety in terms of genotoxicity at concentration 75 mu g/ml and antibacterial activity against Salmonella at all higher concentrations. A final in silico prediction of skin metabolism of compound 3 seems promising, indicating stability of the xanthone moiety in the metabolism process.

Comprehensive phenotypic and genotypic characterization and comparison of virulence, biofilm, and antimicrobial resistance in urinary Escherichia coli isolated from canines

VETERINARY MICROBIOLOGY

Authors: Gilbertie, Jessica M.; Levent, Gizem; Norman, Keri N.; Vinasco, Javier; Scott, H. Morgan; Jacob, Megan E.

Urinary tract infections (UTIs) affect nearly half of women and an estimated 14 % of the canine companion animal population at least once in their lifetime. As with humans, Escherichia coli is the most commonly isolated bacteria from canine UTIs and infections are dominated by specific phylogenetic groups with notable virulence attributes. In this study, we evaluated uropathogenic E. coli (UPEC) (n = 69) isolated from canine UTIs phenotypically and genotypically for virulence factors, biofilm formation and antimicrobial resistance profiles. Biofilm formation in UPEC strains was positively associated with common virulence factors including papG (p = 0.006), fimH (p < 0.0001), sfaS (p = 0.004), focA (p = 0.004), cnf-1 (p = 0.009) and hlyA (p = 0.006). There was a negative association between biofilm formation and phenotypic antimicrobial resistance for ampicillin (p < 0.0004), ciprofloxacin (p < 0.0001), and trimethoprim-sulfamethoxazole (p < 0.02), as well as multidrug resistance (isolates resistant to >= 3 classes of antimicrobials) (p < 0.0002), and the presence of extended spectrum beta-lactamase (ESBL)-producing genes (p < 0.05). In conclusion, UPECs isolated from clinical cases of canine UTIs show a broad negative association between antimicrobial resistance and biofilm formation, and this observation is supported both by phenotypic and genotypic endpoints. As the biofilm formation may result in antimicrobial tolerance, this could be a secondary evasive tactic of UPEC lacking traditional antimicrobial resistance traits. This observation is important for veterinary practitioners to consider when treating puzzling chronic intractable and/or recurrent cases of UTI that appear to be susceptible to antimicrobial therapy via traditional antimicrobial susceptibility testing (AST) methods.

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