Toxoplasma gondii IgM ELISA Kit

Sample

serum

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The Toxoplasma gondii IgM Enzyme Immunoassay Kit provides materials for the quantitative and qualitative determination of IgM-class antibodies to Toxoplasma gondii in human serum and plasma (EDTA, lithium heparin or citrate plasma).
This assay is intended for research use only.

Contents of Kit

1. Microtiterwells, 12×8 (break apart) strips, 96 wells; Wells coated with inactivated Toxoplasma gondii soluble antigen (strain RH). (incl. 1 cover foil)
2. Sample Diluent *, 1 vial, 100 mL, ready to use, colored yellow; pH 7.2 ± 0.2.
3. IgG-RF-Sorbent*, 1 vial, 6.5 mL, ready to use, colored yellow; Contains anti-human IgG-class antibody.
4. Standard (Standard 1-3)*, 3 vials, ready to use;
Concentrations: 25, 50, 100 DU/mL,
Standard 1, 2.0 mL, colored green, green cap
Standard 2, 1.0 mL, colored blue, blue cap
Standard 3, 1.0 mL, colored red, red cap
5. Neg. Control *, 1 vial, 2.0 mL, ready to use; colored yellow, yellow cap.
6. Pos. Control *, 1 vial, 1.0 mL, ready to use; colored purple, black cap.
7. Enzyme Conjugate *, 1 vial, 20 mL, ready to use, antibody to human IgM conjugated to horseradish peroxidase; colored red
8. Substrate Solution, 1 vial, 14 mL, ready to use, Tetramethylbenzidine (TMB).
9. Stop Solution, 1 vial, 14 mL, ready to use, contains 0.2 mol/L H₂SO₄, Avoid contact with the stop solution. It may cause skin irritations and burns.
10. Wash Solution *, 1 vial, 30 mL (20× concentrated for 600 mL), pH 6.5±0.1.
* contain non-mercury preservative

Storage

When stored at 2°C to 8°C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
Opened reagents must be stored at 2°C to 8°C. Microtiter wells must be stored at 2°C to 8°C. Once the foil bag has been opened, care should be taken to close it tightly again.
Opened kits retain activity for 8 weeks if stored as described above.

Detection Range

The range of the assay is between 2.182 DU/mL-100 DU/mL.