T3 (Triiodothyronine) ELISA Kit

Sample

Serum, plasma, cell culture supernatant and other biological samples

Detection Method

cELISA

Intended Use

In vitro quantitative determination of T3 concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

1. ELISA Microplate(Dismountable), 8×12, Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 6 month at -20°C.
2. Lyophilized Standard, 2vial, Put the lyophilized Standard and Biotin-labeled Antibody (lyophilized) into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 6 month at -20°C.
3. Biotin-labeled Antibody (Lyophilized), 1vial, Put the lyophilized Standard and Biotin-labeled Antibody (lyophilized) into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 6 month at -20°C.
4. HRP-Streptavidin Conjugate(SABC, 100×), 120ul, 2-8°C (Avoid Direct Light)
5. TMB Substrate, 10ml, 2-8°C (Avoid Direct Light), 2-8°C (Avoid Direct Light)
6. Purified water, 200ul, 2-8°C
7. Sample Dilution Buffer, 20ml, 2-8°C
8. Antibody Dilution Buffer, 10ml, 2-8°C
9. SABC Dilution Buffer, 10ml, 2-8°C
10. Stop Solution, 10ml, 2-8°C
11. Wash Buffer(25×), 30ml, 2-8°C
12. Plate Sealer, 5 pieces
13. Product Description, 1 copy
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution.

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Performance Characteristics

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

0.156-10ng/ml

Sensitivity

0.094ng/ml

General Description

T3 (Triiodothyronine) is a hormone synthesized and secreted by the thyroid gland, and it is also a type of thyroid hormone. T3 is a biologically active hormone that can affect various processes in the body such as metabolism, growth, and development. It can promote protein synthesis, bone growth and maturation, stimulate the development and maturation of the central nervous system, promote the normal functioning of the cardiovascular system, and regulate body temperature, heart rate, and muscle function. The production of T3 mainly relies on the conversion of the thyroid hormone precursor T4, and most of the T3 is produced by the deiodination of T4 in various tissues and organs. T3 has a wide range of effects on the human body, and both excessive and insufficient secretion of T3 by the thyroid gland can have negative impacts on normal body functions, leading to diseases such as hyperthyroidism and hypothyroidism.

Standard Curve

The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.