Simian Anti-Poliovirus Type 1 IgG ELISA Kit

Sample

Serum

Species Reactivity

Simian

Intended Use

This kit is intended to detect anti-Poliovirus Type 1 IgG antibody in simian serum post vaccination.

Storage

All reagents should be stored at 2-8°C , and should not be used beyond the expiration date on the label. Once opened, microtitration strips may be stored at 2-8°C until the expiration date on the label, provided that desiccated conditions are maintained. Unused strips should be returned to their original foil pouch along with the sachet of desiccant. Opened pouches should be securely resealed by folding over the open end and securing it with adhesive tape.
The working strength Wash Buffer should not be stored for longer than 3 weeks at 2-8°C. It is recommended that Wash buffer be freshly diluted before each assay. If the working strength buffer becomes visibly cloudy or develops precipitate during the 3 weeks, do not use it.
Indications of Deterioration
The Simian Anti-Poliovirus Type 1 Assay may be considered to have deteriorated if:
1. The kit fails to meet the required criteria for a valid test (see Quality Control).
2. Reagents becoming visibly cloudy or develop precipitate. Note: Concentrated Wash buffer, when cold, normally develops crystalline precipitates, which re-dissolve on heating at 37°C.
3. The Substrate Solution turns dark green. This is likely to be caused by contamination of the Substrate Solution.

General Description

Poliomyelitis, often referred to as infantile paralysis or polio, is an infectious disease caused by poliovirus. This acute viral infectious disease is typically spread through two methods, a fecal to oral or oral to oral transmission. Varying types of paralysis may present depending on which nerve is involved.
Poliomyelitis is a term used to identify three different serotypes of disease; these include type 1 (Brunhilde), type 2 (Lansing), and type 3 (Leon). Two types of vaccines were developed to combat the disease. The Salk method, the inactivated poliovirus vaccine (IPV), is based on the 3 wild, virulent reference strains (1, 2, and 3) of poliovirus grown in monkey kidney cell culture (vero cell line) which were inactivated with formalin. The second vaccine is the oral polio vaccine (OPV). This is an inexpensive, attenuated poliovirus, which replicates efficiently in the gut (the primary replication site of the wildtype strain) however is unable to replicate within nervous system tissue. Antibodies should be detectable in animals post vaccination.