Sheep Sterile Filtered Serum (DAGC295)

Product Overview
The raw materials for these products were collected from sheep in the USA in abattoirs registered with the USDA. The animals received ante and post-mortem inspections under a veterinarian's supervision and were apparently free from infectious and contagious diseases. At no time during collection or processing was the material commingled with any other material of animal origin.
Sheep Serum
Alternative Names
Sheep Serum; Serum
0.2 micron (Absolute)
Hemoglobin: <30 mg/dl
Protein:>5.5 g/dl
100ml, 500ml
pH: 6.8 - 8.2
This serum is stable for ≥ 3 years when stored −20°C ± 5°C. Avoid repeated freezing and thawing.
Sheep Serum; Serum


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A universal assay strategy for sensitive and simultaneous quantitation of multiplex tumor markers based on the stirring rod-immobilized DNA-LaMnO3 perovskite-metal ions encoded probes


Authors: Wang, Wenhai; Wang, Qiqin; Xie, Hongzhen; Wu, Dazhen; Gan, Ning

It was extremely urgent to develop some simultaneous and sensitive biosensors for detecting multiplex serum tumor markers (TMs) for early screening of cancers. Herein, a multiplex assay was developed based on the DNA-LaMnO3 (DNA-LMO) perovskite encoded probes and targets mediated competitive replacement strategy. Alpha fetoprotein (AFP), carcinoembryonic antigen (CEA) and prostate specific antigen (PSA) markers were employed as representative target TMs. Aptasensor is prepared by a series of DNA-LMO-M encode probes which were prepared by three hyperbranched DNA firstly immobilized on LMO encapsulating Pb, Cd or Cu ions. Then, three TMs aptamers were labeled on the stirring-rod and hybridized with the probes. After the developed encoded probes was incubated the TMs, the encoded probes corresponding to different TMs can be released into the supernatant through the competitive replacement. The inner metal ion can be simultaneously detected by square wave voltammetry corresponding to various TMs. Since the stirring rod can enrich many encoded probes containing a lot of metal ions, multiplex signal amplification can be realized. Due to the enrichment and easy separation of the stirring rod, the signal-to-noise ratio was also obviously improved and thus to results in good sensitivity and accuracy. Moreover, it took only 20 min to detect three targets which much faster than many same types of aptasensor. Under the optimal conditions, the low detection limit for CEA (3.6 x 10(-4) ng/mL), AFP (3.4 x 10(-4) ng/mL) and PSA (2.8 x 10(-4) ng/mL) were obtained. Therefore, this method is likely to be used for early and sensitive screening of tumors.

Repeated and single maternal separation specifically alter microglial morphology in the prefrontal cortex and neurogenesis in the hippocampus of 15-day-old male mice


Authors: Reshetnikov, Vasiliy; Ryabushkina, Yulia; Kovner, Anna; Lepeshko, Arina; Bondar, Natalia

Early-life adversity impairs neuronal plasticity of the developing brain. In rodents, brain maturation processes, including neuro- and synaptogenesis, myelination, microglial maturation, and hypothalamic-pituitary-adrenal (HPA) axis development continue in the postnatal period. In our study, two models of early-life stress were used: repeated maternal separation (MS) from postnatal day (PND) 2 to PND14 for 3 h daily and single maternal deprivation (MD) on PND9 for 24 h. Effects of each type of early-life stress on neuron density, neurogenesis, microglial morphology, and HPA axis programming were studied in 15-day-old male mice. Neither early-life stress paradigm affected the expression of stress-related genes (Crh, Avp, Crhr1, Crhr2, Nr3c1, and Nr3c2) and the serum level of corticosterone on PND15. Immunohistochemical analysis was performed on slices of the hippocampus and prefrontal cortex (PFC) with antibodies against a marker of mature neurons (NeuN), of microglia (Iba1), proliferating cells (Ki67), and immature neurons (DCX). We found higher density of ameboid microglia and intermediate microglia in the PFC in groups MS and MD, respectively, than in a control group. In both stressed groups, a higher number of Ki67-positive cells was noted in the dentate gyrus. Thus, in mice, the process of transformation of ameboid microglia into ramified ones as well as a neurogenesis reduction take place during the second postnatal week, whereas early-life stress can disturb these processes in a stress- and region-dependent manner.

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