SARS S protein (R667A) [His] (DAGC211)

Recombinant SARS S protein (R667A) from HEK293 Cells [His]

Product Overview
SARS S protein (R667A), His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A)). This protein carries a polyhistidine tag at the C-terminus.
Predicted N terminal
Ser 14
Molecular Weight
The protein has a calculated MW of 133.0 kDa. The protein migrates as 120-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per ug by the LAL method.
Alternative Names
SARS; SARS Spike Protein; SARS S Protein
>95% as determined by SDS-PAGE.
100ug, 1mg
Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
SARS; SARS Spike Protein; SARS S Protein

Data Examples

SARS S protein (R667A), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.


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Li, SM; Li, GM; et al. Reduced incorporation of SARS-CoV spike protein into viral particles due to amino acid substitutions within the receptor binding domain. JAPANESE JOURNAL OF INFECTIOUS DISEASES 61:123-127(2008).
Zhou, ZM; Post, P; et al. A recombinant baculovirus-expressed S glycoprotein vaccine elicits high titers of SARS-associated coronavirus (SARS-CoV) neutralizing antibodies in mice. VACCINE 24:3624-3631(2006).

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