Product Overview
A DNA sequence encoding the SARS-CoV-2 Spike Protein (RBD) (YP_009724390.1(V367F)) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus.
Target
SARS-CoV-2 Spike RBD
Predicted N terminal
Arg319
Molecular Weight
The recombinant SARS-CoV-2 Spike Protein (RBD, His Tag) consists of 234 amino acids and predicts a molecular mass of 26.59 kDa.
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Alternative Names
SARS-CoV-2; coronavirus; SARS-CoV-2 spike RBD; SARS-CoV-2 spike protein
Purity
> 95 % as determined by SDS-PAGE.
Buffer
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Storage
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Introduction
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process.
Keywords
SARS-CoV-2; coronavirus; SARS-CoV-2 spike RBD; SARS-CoV-2 spike protein
Datasheet