SARS-CoV-2 Spike RBD (E484K) [rFc] (DAGC603)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Product Overview
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD (YP_009724390.1, with mutation E484K) (Arg319-Phe541) was expressed with the Fc region of rabbit IgG1 at the C-terminus.
Target
SARS-CoV-2 Spike RBD
Nature
Recombinant
Predicted N terminal
Arg319
Tag/Conjugate
rFc
Molecular Weight
The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD consists of 448 amino acids and predicts a molecular mass of 50.4 kDa. As a result of glycosylation, it migrates as an approximately 53.5 kDa band in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Alternative Names
SARS-CoV-2 Spike RBD; SARS-CoV-2; SARS-CoV-2 S1 RBD; SARS-CoV-2 Spike; SARS-CoV-2 RBD
Procedure
None
Purity
> 90 % as determined by SDS-PAGE
Format
Lyophilized
Size
100ug
Buffer
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Preservative
None
Storage
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Introduction
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.
Keywords
SARS-CoV-2 Spike RBD; SARS-CoV-2; SARS-CoV-2 S1 RBD; SARS-CoV-2 Spike; SARS-CoV-2 RBD

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