Serum, plasma, whole blood
The kit has been verified by high purity SARS-CoV-2 (2019-nCoV) Nucleoprotein/NP Protein. It can recognize recombinant SARS-CoV Nucleoprotein/NP Protein (His Tag), but no react with recombinant MERS-CoV Nucleoprotein/NP protein (His Tag).
Store at 2 - 8°C and the kit is stable for 6 months upon receipt.
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.