SARS-CoV-2 Nucleoprotein ELISA Kit (DEIASL017)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
Serum, plasma, whole blood
Intended Use
The kit has been verified by high purity SARS-CoV-2 (2019-nCoV) Nucleoprotein/NP Protein. It can recognize recombinant SARS-CoV Nucleoprotein/NP Protein (His Tag), but no react with recombinant MERS-CoV Nucleoprotein/NP protein (His Tag).
Storage
Store at 2 - 8°C and the kit is stable for 6 months upon receipt.
General Description
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

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References


Breastfed 13 month-old infant of a mother with COVID-19 pneumonia: a case report

INTERNATIONAL BREASTFEEDING JOURNAL

Authors: Yu, Yuanyuan; Li, Youjiang; Hu, Yingying; Li, Bin; Xu, Jian

Background In China, mothers with confirmed or suspected COVID-19 pneumonia are recommended to stop breastfeeding. However, the evidence to support this guidance is lacking. There have been relatively few cases reported about direct breastfeeding an infant by a mother with SARS-CoV-2 pneumonia. Therefore, it is necessary to assess the safety of breastfeeding and the possible protective effects of breast milk on infants. Case presentation This report analyzes the case of a mother who continued breastfeeding her 13 month-old child when both were diagnosed with confirmed COVID-19 pneumonia. We describe the clinical presentation, diagnosis, treatment, and outcome. The presence of SARS-CoV-2 nucleic acid was determined in maternal serum, breast milk, nasopharyngeal (NP) swabs and feces, and in infant serum, NP swabs and feces. IgM and IgG antibodies against SARS-CoV-2 were assessed in maternal serum and breast milk and in infant serum. SARS-CoV-2 nucleic acid was not detected in the breast milk, and antibodies against SARS-CoV-2 were detected in the mother's serum and milk. Conclusions The present case further confirms that the possibility of mother-to-child transmission about SARS-CoV-2 via breast milk alone was very small, and breast milk is safe for direct feeding of infants.

False-positive SARS-CoV-2 serology in 3 children with Kawasaki disease

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE

Authors: To, Kelvin K. W.; Chua, Gilbert T.; Kwok, Ka Li; Wong, Joshua S. C.; Au, Dennis Chi Yu; Lam, Yuen Yu; Wong, Wilfred H. S.; Ho, Marco H. K.; Chan, Godfrey C. F.; Chui, Celine S. L.; Li, Xue; Tung, Keith T. S.; Wong, Rosa S.; Tso, Winnie W. Y.; Wong, Ian C. K.; Wong, Christina S. M.; Fong, Carol H. Y.; Chan, Kwok Hung; Yuen, Kwok Yung; Ip, Patrick; Kwan, Mike Y. W.

Background: Kawasaki disease (KD) is an acute febrile and eruptive disease with systemic vasculitis predominantly affecting young East Asian children. Recent reports showed that children with KD-like disease from KD low prevalence regions had positive SARS-CoV-2 serology despite a negative SARS-CoV-2 polymerase chain reaction (PCR) in respiratory samples. Objectives: To describe 3 pediatric Kawasaki Disease patients with false positive SARS-CoV-2 serology. Study design: We retrospectively recruited children with KD diagnosed during the COVID-19 outbreak in Hong Kong. Clinical characteristics and laboratory test results including SARS-CoV-2 PCR results were retrieved. We performed a microparticle-based immunoassay for the detection of IgG against nucleoprotein (NP) and spike protein receptor binding domain (RBD), and a microneutralization assay for the detection of neutralizing antibodies. Results: Three Chinese children with typical KD were identified. They had no epidemiological links with COVID-19 patients and tested negative for SARS-CoV-2 NPA PCR. Theywere treated with IVIG and aspirin, and were discharged without complications. Subsequently 2 of them were tested positive against anti-RBD and anti-NP antibodies and 1 was tested positive against anti- RBD antibodies. However, microneutralization assay showed that neutralizing antibodies were absent, suggesting a false-positive IgG result. Conclusion: Detection of neutralizing antibodies is recommended to confirm previous SARS-CoV-2 infection in IgGpositive but PCR-negative patients. (C) 2020 The Author(s). Published by Elsevier Inc.

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