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Salmonella Enteritidis (SE) is a major bacterial pathogen affecting the poultry industry worldwide, causing significant economic losses due to reduced productivity, increased mortality, and the need for costly control measures. Additionally, SE is a zoonotic agent, capable of transmitting from poultry to humans through the consumption of contaminated eggs, meat, or other poultry products, leading to foodborne illnesses. Accurate and timely detection of SE in poultry flocks is crucial for effective disease management, outbreak control, and ensuring food safety. The Salmonella Enteritidis ELISA Kit is a specialized diagnostic tool designed to meet the demands of poultry disease research and vaccine monitoring, providing a reliable, sensitive, and efficient method for the detection of SE antigens or antibodies.
Figure 1. Salmonella.
Technology Platform
Sample Type Compatibility
Sensitivity Profile
Kit Features
Among numerous serotypes of Salmonella, Salmonella Enteritidis (SE) has attracted much attention due to its ability to infect poultry, especially laying hens, and the resulting food safety risks. Unlike many other Salmonella bacteria, SE can vertically spread into eggs without causing obvious clinical symptoms in poultry. This makes it one of the main causes of human Salmonella infections worldwide. The impact on poultry including:
Performance Parameters
Sample Types
Data Analysis
Applications in Poultry Disease Research
Evaluating Vaccine Uniformity: A successful vaccination program requires that a high percentage of the birds in the flock mount a uniform immune response. ELISA testing can identify variations in antibody titers across the flock. Significant variations may point to issues such as uneven vaccine delivery, differences in bird health, or sub-optimal vaccination techniques, all of which can be addressed to improve flock health and protection.
Monitoring Long-Term Immunity: The protective immunity provided by a vaccine can wane over time. Regular serological surveillance using the ELISA kit allows producers to monitor the durability of the immune response. If antibody titers begin to drop below a protective threshold, it may be an indication that a booster vaccination is required, ensuring that the flock remains protected throughout its production cycle.
| Parameter | Details |
| Product name | Chicken Salmonella Enteritidis IgY ELISA Kit |
| Sample | Serum, Plasma and other biological fluids |
| Intended Use | The Chicken Salmonella Enteritidis IgY (CSE-IgY) ELISA Kit is to be used for the vitro quantitative determination of Chicken CSE-IgY in Serum, Plasma and other biological fluids. The Kit is intended for research use only, not for diagnostic or therapeutic procedure. |
| Storage | Intra-Assay: CV ≤ 15% Inter-Assay: CV ≤ 15% Correlation coefficient (R) of linear regression of the samples is more than 0.92. |
High Specificity
The kit employs antibodies that specifically recognize SE antigens or antigens that specifically bind to anti-SE antibodies, minimizing cross-reactivity with other Salmonella serotypes or non-Salmonella bacteria. This ensures accurate identification of SE, reducing the risk of false-positive results.
High Sensitivity
It can detect low concentrations of SE antigens or antibodies, enabling early detection of infections or immune responses. This is particularly important for monitoring the early stages of disease development or evaluating the efficacy of vaccines, where low levels of the target analyte may be present.
Easy to Use
The assay protocol is straightforward and requires minimal technical expertise. The kit comes with detailed instructions, and the steps (sample addition, incubation, washing, substrate addition, and measurement) can be easily performed in a standard laboratory setting.
The Salmonella Enteritidis ELISA Kit is an indispensable tool for poultry disease research and vaccine monitoring, offering high specificity, sensitivity, rapid results, and ease of use. By enabling accurate detection and quantification of SE antigens or antibodies, it contributes to effective disease management, improved vaccine development, and enhanced food safety. Whether in research laboratories, diagnostic facilities, or poultry farms, this kit provides reliable and valuable data to support the fight against Salmonella Enteritidis infections in poultry.
The kit can typically detect IgY antibodies within a range of 5 - 500 ng/mL (or equivalent U/mL based on the standard), with a lower limit of detection (LoD) of <2 ng/mL.
Yes, with validation. The kit is primarily validated for chicken serum and plasma. However, researchers have successfully adapted it for testing egg yolk extracts to monitor flock immunity non-invasively. We recommend running a small validation study to confirm matrix effects for non-standard samples.
This is a crucial point. Our kit detects antibodies against specific SE antigens. If the vaccine used is based on the same antigens (e.g., outer membrane proteins), the ELISA will not differentiate. For Differential Diagnostic ELISA (DIVA), please inquire about our specific SE-DIVA ELISA Kit.
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