Salicylate Assay Kit

Sample

Rat plasma, Rat serum, Human serum, Human plasma, and Human urine

Detection Method

Colorimetric Tests

Intended Use

The Salicylate Assay Kit provides a convenient and reliable means to measure salicylate. In the assay, salicylate complexes with ferric chloride to create a colored compound that can be measured at 560 nm. This assay can be used with a variety of samples and is simple, sensitive, and adaptable to high-throughput screening.

Contents of Kit

The kit is sufficient for 100 colorimetric assays in 96-well plates.
• Reagent 20 mL
• Standard (100 mM Salicylate) 200 μL

Storage

The kit is shipped at room temperature. Store components at 2-8 °C.

General Description

Salicylate is a salt or ester of salicylic acid and can be found naturally in some plants. It is also a metabolic byproduct of aspirin (acetylsalicylic acid) and salicylate concentrations are often tested in blood or urine in cases of suspected overdose. Salicylic acid is commonly used in skincare products as an exfoliating ingredient, and in other consumer products as a preservative.
The Salicylate Assay Kit provides a convenient and reliable means to measure salicylate. In the assay, salicylate complexes with ferric chloride to create a colored compound that can be measured at 560 nm. This assay can be used with a variety of samples and is simple, sensitive, and adaptable to high-throughput screening.
The linear detection range of the kit is 0.8 mM (10.9 mg/dL) to 20 mM (274.2 mg/dL) salicylate The kit is suitable for salicylate activity determination in biological samples such as serum, plasma, urine, as well as for consumer products e.g., beauty products and mouthwash.
Salicylate was spiked into rat plasma, rat serum, human serum, and human plasma, and human urine, and was assayed using the 96-well plate assay protocol and was determined to have acceptable % recovery. EDTA, Heparin, Citrate, and RIPA buffer do not interfere with this assay. Beauty products and mouthwash are also compatible with this kit.