RSV Pre-F (B) ELISA Kit (Φ、V)

Name: RSV Pre-F (B) ELISA Kit (Φ、V)
SKU: DEIA-NS251002
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Biological samples

Species Reactivity

RSV

Detection Method

sELISA

Intended Use

This kit uses a sandwich enzyme-linked immunosorbent assay (ELISA) to detect RSV B Pre-F. The kit is coated with an antibody targeting the Φ site, and the detection antibody targets the V site, allowing for highly sensitive and specific detection and quantitative analysis of RSV Pre-F content.

Contents of Kit

1. RSV Pre-F Microplate, Pre-coated with capture antibody, 96 T/Kit
2. RSV Pre-F Detection Antibody, HRP-labeled detection antibody, Dilution 1:50, 200μl
3. RSV Pre-F Standard, Recombinant RSV Pre-F, 10μg/ml, 250μl
4. 20×Wash Buffer, 20× Wash Concentrate, 30ml
5. Dilution Buffer 1, Sample Dilution Buffer, 30ml
6. Dilution Buffer 2, Enzyme Dilution Buffer, 15ml
7. Color Reagent A, Substrate Solution A, 6ml
8. Color Reagent B, Substrate Solution B, 6ml
9. Stop Reagent, Stop Solution, 6ml
10. Sealing Film, Plate Sealer, 2 pieces
Note: Components from different batches should not be mixed.

Storage

Unopened kit: Store desiccated and protected from light at 2-8°C. The validity period is 12 months.
After opening the kit, please use it as soon as possible.

Precision

The intra-assay precision (CV) is ≤ 15%.

Detection Range

The detection range of the kit is 7.8-250 ng/ml.

Sensitivity

The sensitivity of the kit is 3.9 ng/ml.
The Limit of Blank of the kit is ≤ 0.1.

General Description

Respiratory syncytial virus (RSV) is one of the most important pathogens causing acute lower respiratory tract infections in infants, young children, the elderly, and immunocompromised individuals. The fusion protein (F protein) on the virus surface is a highly conserved and highly antigenic type I transmembrane glycoprotein, existing in two conformations: pre-fusion (Pre-F) and post-fusion (Post-F). The Pre-F protein contains six antigenic sites, among which the antigenic site Φ is unique to the Pre-F protein, and antibodies induced against this site have the highest neutralizing activity. This kit uses a sandwich enzyme-linked immunosorbent assay (ELISA) to detect RSV B Pre-F. The kit is coated with an antibody targeting the Φ site, and the detection antibody targets the V site, allowing for highly sensitive and specific detection and quantitative analysis of RSV Pre-F content.

Standard Curve

Example:
The following standard curve is for reference only. The standard curve generated in the same experiment should be used to calculate sample concentrations.