5X Reaction Buffer: 250 mM Tris-HCl, pH 8.3, + 375 mM KCl + 15 mM MgCl 2 + 50 mM DTT.
Preservative
None
Storage
2-8°C short term, -20°C long term
Introduction
The murine leukemia viruses (MLVs or MuLVs) are retroviruses named for their ability to cause cancer in murine (mouse) hosts. Some MLVs may infect other vertebrates. MLVs include both exogenous and endogenous viruses. Replicating MLVs have a positive sense, single-stranded RNA (ssRNA) genome that replicates through a DNA intermediate via the process of reverse transcription.
Keywords
MMLV reverse transcriptase; Murine leukemia virus; Gammaretrovirus; MLVs; MuLVs; Reverse transcriptase; RVT_1; EC: 2.7.7.49; RNA-dependent DNA polymerase; DNA polymerase enzyme; M-MLV reverse transcriptase; Moloney murine leukemia virus; Moloney Mouse Leu
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