Molecular Weight
37.5 kDa
Alternative Names
Clumping factor A; Fibrinogen receptor A; Fibrinogen-binding protein A; clfA; SACOL0856
Purity
> 90% as determined by SDS-PAGE
Format
Liquid or Lyophilized powder
Concentration
Batch dependent - please inquire should you have specific requirements.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Introduction
Staphylococcus aureus Clumping Factor A (ClfA) is a surface-exposed virulence protein of S. aureus, belonging to the MSCRAMM adhesin family. It mediates bacterial attachment to host tissues by binding fibrinogen via a "dock, lock, latch" mechanism, crucial for immune evasion and biofilm formation. ClfA facilitates bloodstream infections, endocarditis, and medical device colonization by enabling bacterial clumping under physiological shear stress. Its fibrinogen-binding domain (residues 221–550) is a prime target for therapeutic antibodies and vaccines against antibiotic-resistant strains, including MRSA.
Keywords
Staphylococcus aureus Clumping factor A; Fibrinogen receptor A; Fibrinogen-binding protein A; clfA; SACOL0856
Citations
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