Product Overview
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Molecular Weight
30.4 kDa
Alternative Names
Enterotoxin type E; SEEB; entE
Purity
> 90% as determined by SDS-PAGE
Format
Liquid or Lyophilized powder
Concentration
Batch dependent - please inquire should you have specific requirements.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Introduction
Staphylococcus aureus Enterotoxin Type E (SEE) is a heat-stable exotoxin (molecular weight: ~28.4 kDa) produced by Staphylococcus aureus and classified among the "classical" enterotoxins (SEA-SEE). As a superantigen, SEE bypasses conventional antigen processing by binding directly to MHC class II molecules and specific T-cell receptor regions, triggering massive cytokine release (e.g., TNF-α, IL-6) that can lead to toxic shock syndrome or food poisoning. SEE is highly resistant to heat (surviving 100°C for 30 minutes) and digestive proteases (e.g., pepsin), allowing it to persist in contaminated foods. Methods include multiplex PCR for gene identification (entE) and ultra-sensitive ELISA/LC-MS/MS.
Keywords
Staphylococcus aureus Enterotoxin type E; SEE; entE
Citations
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