Product Overview
E. coli derived, Gln26-Arg170, with an N terminal Met
Tag/Conjugate
Unconjugated
Molecular Weight
17.2 kDa
Bio-activity
Measured in a cell proliferation assay using MC/92 mouse mast cells. The ED50 for this effect is 315 ng/mL.
Endotoxin
<1.0 EU per 1 μg of the protein by the LAL method.
Alternative Names
Interleukin 10; IL10; EpsteinBarr virus; EBV; EBV IL10; IL-10
Purity
>97%, by SDSP AGE under reducing conditions and visualized by silver stain
Buffer
Lyophilized from 40.3 μL of a 0.2 μm filtered solution in 20 mM Tris and 150 mM NaCl, pH 8.0 with 50 μg BSA per 1 μg as a carrier protein
Storage
Store at -20°C to -70°C
Reconstitution
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Ship
The product is shipped at ambient temperature.
Introduction
Interleukin 10 (IL10) is a pleiotropic cytokine that plays an important role in regulating inflammatory and immune responses. It is a potent suppressant of proinflammatory cytokine production by monocytes/macrophages and neutrophils. It is also an inhibitor of macrophage and T cell effector functions. Homologs of mammalian IL10 have been identified in the genome of several viruses, including EpsteinBarr virus (EBV), poxvirus Orf, baboon cytomegalovirus, and human and equine herpes virus. In the EBV genome, the BCRF1 open reading frame encodes the EBV IL10 (vIL10). The viral IL10 precursor is a 170 amino acid residue (aa) protein with a putative 25 aa signal peptide that is cleaved to yield the 145 aa mature protein. The EBV IL10 precursor shares approximately 78% and 65% amino acid
sequence homology with human and mouse IL10, respectively. Most of the deviations are localized to the signal peptide and the first 20 aminoterminal residues. Viral IL10 is expressed during the late phase of the lytic cycle of EBV infection. Viral IL10 is a partial agonist of mammalian IL10 and shares many of their activities. It is likely that vIL10 may have a role in host immune evasion.
Keywords
Interleukin 10; IL10; EpsteinBarr virus; EBV; EBV IL10; EBV IL-10; IL-10
Citations
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