E. coli derived, Gln26-Arg170, with an N terminal Met
Measured in a cell proliferation assay using MC/92 mouse mast cells. The ED50 for this effect is 315 ng/mL.
<1.0 EU per 1 μg of the protein by the LAL method.
Interleukin 10; IL10; EpsteinBarr virus; EBV; EBV IL10; IL-10
>97%, by SDSP AGE under reducing conditions and visualized by silver stain
Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with BSA as a carrier protein
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -70°C under sterile conditions after reconstitution.
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
The product is shipped at ambient temperature.
Interleukin 10 (IL10) is a pleiotropic cytokine that plays an important role in regulating inflammatory and immune responses. It is a potent suppressant of proinflammatory cytokine production by monocytes/macrophages and neutrophils. It is also an inhibitor of macrophage and T cell effector functions. Homologs of mammalian IL10 have been identified in the genome of several viruses, including EpsteinBarr virus (EBV), poxvirus Orf, baboon cytomegalovirus, and human and equine herpes virus. In the EBV genome, the BCRF1 open reading frame encodes the EBV IL10 (vIL10). The viral IL10 precursor is a 170 amino acid residue (aa) protein with a putative 25 aa signal peptide that is cleaved to yield the 145 aa mature protein. The EBV IL10 precursor shares approximately 78% and 65% amino acid
sequence homology with human and mouse IL10, respectively. Most of the deviations are localized to the signal peptide and the first 20 aminoterminal residues. Viral IL10 is expressed during the late phase of the lytic cycle of EBV infection. Viral IL10 is a partial agonist of mammalian IL10 and shares many of their activities. It is likely that vIL10 may have a role in host immune evasion.
Interleukin 10; IL10; EpsteinBarr virus; EBV; EBV IL10; EBV IL-10; IL-10