Recombinant EBV IL-10 Protein (DAGC009)

Recombinant Epstein Barr virus IL-10 Protein from E.coli

Product Overview
E. coli ­derived, Gln26-­Arg170, with an N ­terminal Met
Nature
Recombinant
Tag/Conjugate
Unconjugated
Molecular Weight
17.2 kDa
Bio-activity
Measured in a cell proliferation assay using MC/9­2 mouse mast cells. The ED50 for this effect is 3­15 ng/mL.
Endotoxin
<1.0 EU per 1 μg of the protein by the LAL method.
Alternative Names
Interleukin 10; IL­10; Epstein­Barr virus; EBV; EBV IL­10; IL­-10
Purity
>97%, by SDS­P AGE under reducing conditions and visualized by silver stain
Format
Lyophilized
Size
10 ug
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with BSA as a carrier protein
Preservative
None
Storage
12 months from date of receipt, -­20 to -­70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -­70°C under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Ship
The product is shipped at ambient temperature.
Introduction
Interleukin 10 (IL­10) is a pleiotropic cytokine that plays an important role in regulating inflammatory and immune responses. It is a potent suppressant of proinflammatory cytokine production by monocytes/macrophages and neutrophils. It is also an inhibitor of macrophage and T cell effector functions. Homologs of mammalian IL­10 have been identified in the genome of several viruses, including Epstein­Barr virus (EBV), poxvirus Orf, baboon cytomegalovirus, and human and equine herpes virus. In the EBV genome, the BCRF1 open reading frame encodes the EBV IL­10 (vIL­10). The viral IL­10 precursor is a 170 amino acid residue (aa) protein with a putative 25 aa signal peptide that is cleaved to yield the 145 aa mature protein. The EBV IL­10 precursor shares approximately 78% and 65% amino acid
sequence homology with human and mouse IL­10, respectively. Most of the deviations are localized to the signal peptide and the first 20 amino­terminal residues. Viral IL­10 is expressed during the late phase of the lytic cycle of EBV infection. Viral IL­10 is a partial agonist of mammalian IL­10 and shares many of their activities. It is likely that vIL­10 may have a role in host immune evasion.
Keywords
Interleukin 10; IL­10; Epstein­Barr virus; EBV; EBV IL­10; EBV IL­-10; IL­-10

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