Recombinant ASFV P30 Protein [His] (DAGC007)

Recombinant African Swine fever virus P30 Protein from E.coli [His]

Product Overview
Recombinant African Swine Fever virus protein was expressed in E.coli as a his-tag fusion protein (210 aa,>95%, ~24.4 kda).
Specificity
African Swine Fever Virus (ASFV, 210-aa, protein accession # P34204-1) is 100% conserved in African Swine Fever virus.
Nature
Recombinant
Tag/Conjugate
His
Molecular Weight
24.4 kDa
Endotoxin
< 1.0 EU per µg protein as determined by the LAL method.
Alternative Names
ASFV P30
Format
Liquid
Size
10 µL
Buffer
20mM Tris-HCl (pH:8.0), 0.08M NaCl, 2mM β-mercaptoethanol, 0.2mM EDTA, 2M Guanidine-HCl and 95mM Imidazole
Preservative
None
Storage
Short-term: unopened, undiluted vials for less than a week at 4°C. Long-term: at –20°C or below in suitable aliquots after
reconstitution. Do not freeze and thaw and store working, diluted solutions.
Ship
4°C for solutions and room temperature for lyophilized powder.
Introduction
African swine fever virus (ASFV) is a large, double-stranded DNA virus in the Asfarviridae family. It is the causative agent of African swine fever (ASF). The virus causes a haemorrhagic fever with high mortality rates in domestic pigs; some isolates can cause death of animals as quickly as a week after infection. It persistently infects its natural hosts, warthogs, bushpigs, and soft ticks of the genus Ornithodoros, which likely act as a vector, with no disease signs. It does not cause disease in humans. ASFV replicates in the cytoplasm of infected cells. It is the only known virus with a double-stranded DNA genome to be transmitted by arthropods.
Keywords
African Swine fever virus; ASFV; ASFV P30

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References


Potential use of oral fluid samples for serological diagnosis of African swine fever

VETERINARY MICROBIOLOGY

Authors: Mur, Lina; Gallardo, Carmina; Soler, Alejandro; Zimmermman, Jeffrey; Pelayo, Virginia; Nieto, Raquel; Manuel Sanchez-Vizcaino, Jose; Arias, Marisa

African swine fever (ASF) is a complex, highly lethal, notifiable disease of swine. ASF is wide-spread in sub-Saharan Africa and East European countries and there is presently a great risk of spread to neighboring countries. Since there is no vaccine for ASF virus (ASFV), control is based on rapid and early detection of the disease via surveillance. This approach requires collecting blood samples from large number of animals. Laborious and expensive of itself, this process also presents an additional risk because ASFV is present at high concentrations in the blood. The objective of this study was to initiate studies into the potential use of oral fluid as an alternative to serum for ASF diagnosis, for latter studying its possible use in surveillance and control programs. To this end, oral fluid samples collected at different times post infection from eight pigs experimentally inoculated with an attenuated ASFV were assayed using modified protocols of the two validated serological techniques, the enzyme-immune-liked assay (ELISA) and immunoperoxidase technique (IPT). Antibodies against ASFV were detected in oral fluid samples of all animals from early post infection through the end of the experiment by ELISA and IPT. These results confirmed the presence of ASFV antibodies in swine oral fluids samples, the possibility of an oral fluid-based approach in ASF diagnosis and, potentially in ASF surveillance. (C) 2013 Elsevier B.V. All rights reserved.

Antigenic regions of African swine fever virus phosphoprotein P30

TRANSBOUNDARY AND EMERGING DISEASES

Authors: Wu, Ping; Lowe, Andre D.; Rodriguez, Yelitza Y.; Murgia, Maria V.; Dodd, Kimberly A.; Rowland, Raymond R.; Jia, Wei

African swine fever (ASF) is one of the most complex and lethally haemorrhagic viral diseases of swine, affecting all breeds and ages of pigs. In the absence of ASF vaccines, reliable laboratory diagnosis and restricted biosecurity are critical for disease prevention and control. A detection of ASF-specific antibodies in an unvaccinated pig is a good marker for the diagnosis of ASF. The immunoperoxidase test (IPT) is a sensitive test for detecting ASF virus (ASFV) antibodies. However, due to the complexity of the procedure, the IPT is only suitable to be used as a confirmatory test. The ASFV p30 protein-based enzyme-linked immunosorbent assay (ELISA) is widely used for ASFV antibody screening, but the sensitivity is not comparable to the IPT. It is essential to have a better understanding of the antigenic properties of ASFV p30 to improve p30-based serologic tests. In this study, we developed a panel of 21 monoclonal antibodies (mAbs) against ASFV p30. With 14 out of the 21 mAbs, we defined 4 antigenic regions that contain at least 4 linear epitopes. Nine of the 14 mAbs mapped to antigenic regions 3 and 4 reacted with p30 in all serologic methods tested in this study, such as indirect immunofluorescence assay (IFA), ELISA and Western blot. The antigenic regions 3 and 4 are highly conserved and immunodominant in host antibody response. These mAbs and the defined p30 antigenic regions 3 and 4 provide valuable tools for the development and improvement of ASF serologic assays.

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