Recombinant ASFV p30 Protein [His] (DAGPY01015)

Recombinant African Swine Fever Virus p30 Protein from Insect Cells [His]

Product Overview
African Swine Fever Virus (ASFV) p30, Recombinant. Product contains a His tag.
Nature
Recombinant
Tag/Conjugate
His
Molecular Weight
~32 kDa
Alternative Names
ASFV P30
Purity
≥ 85% Purity (SDS-PAGE). Affinity purified.
Format
Liquid
Concentration
(Lot Specific) mg/mL (Bradford)
Size
0.1 mg
Buffer
Phosphate Buffered Saline, pH 7.4
Preservative
0.09% Sodium Azide
Storage
Store at -20°C. Aliquot to avoid multiple freeze/thaw cycles.
Introduction
African swine fever virus (ASFV) is a large, double-stranded DNA virus in the Asfarviridae family. It is the causative agent of African swine fever (ASF). The virus causes a haemorrhagic fever with high mortality rates in domestic pigs; some isolates can cause death of animals as quickly as a week after infection. It persistently infects its natural hosts, warthogs, bushpigs, and soft ticks of the genus Ornithodoros, which likely act as a vector, with no disease signs. It does not cause disease in humans. ASFV replicates in the cytoplasm of infected cells. It is the only known virus with a double-stranded DNA genome to be transmitted by arthropods.
Keywords
African Swine fever virus; ASFV; ASFV P30

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References


Cryo-EM cools down swine fever

JOURNAL OF BIOLOGICAL CHEMISTRY

Authors: Gallagher, John R.; Harris, Audray K.

African swine fever virus (ASFV) is among the most complex DNA viruses known. Outbreaks have killed millions of swine around the world, and there is currently no vaccine. Three recent papers report the cryo-EM structure of the complete ASFV virion, comprising a viral particle of multiple layers, and resolve the major outer-capsid protein p72 to higher resolution. Progress in these reports provides a further understanding of the structure-function relationships of large viruses and should aid in ASFV vaccine development.

Antiviral Role of IFITM Proteins in African Swine Fever Virus Infection

PLOS ONE

Authors: Munoz-Moreno, Raquel; Cuesta-Geijo, Miguel Angel; Martinez-Romero, Carles; Barrado-Gil, Lucia; Galindo, Inmaculada; Garcia-Sastre, Adolfo; Alonso, Covadonga

The interferon-induced transmembrane (IFITM) protein family is a group of antiviral restriction factors that impair flexibility and inhibit membrane fusion at the plasma or the endosomal membrane, restricting viral progression at entry. While IFITMs are widely known to inhibit several single-stranded RNA viruses, there are limited reports available regarding their effect in double-stranded DNA viruses. In this work, we have analyzed a possible antiviral function of IFITMs against a double stranded DNA virus, the African swine fever virus (ASFV). Infection with cell-adapted ASFV isolate Ba71V is IFN sensitive and it induces IFITMs expression. Interestingly, high levels of IFITMs caused a collapse of the endosomal pathway to the perinuclear area. Given that ASFV entry is strongly dependent on endocytosis, we investigated whether IFITM expression could impair viral infection. Expression of IFITM1, 2 and 3 reduced virus infectivity in Vero cells, with IFITM2 and IFITM3 having an impact on viral entry/uncoating. The role of IFITM2 in the inhibition of ASFV in Vero cells could be related to impaired endocytosis-mediated viral entry and alterations in the cholesterol efflux, suggesting that IFITM2 is acting at the late endosome, preventing the decapsidation stage of ASFV.

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