Rat Col27a1 (Collagen alpha-1 (XXVII) chain) ELISA Kit (DEIA-FN303)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma, cell culture supernatants, tissue homogenate
Species Reactivity
Rat
Intended Use
For quantitative detection of Rat Col27a1 (Collagen alpha-1 (XXVII) chain) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
1.56-100 ng/mL
Sensitivity
0.938 ng/mL
Standard Curve

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References


Pace, JM; Corrado, M; et al. Identification, characterization and expression analysis of a new fibrillar collagen gene, COL27A1. MATRIX BIOLOGY 22:3-14(2003).
Plumb, DA; Ferrara, L; et al. Collagen XXVII Organises the Pericellular Matrix in the Growth Plate. PLOS ONE 6:-(2011).

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